Browsing Faculty of Natural Sciences by Author "Tuffin, Marla"
Now showing items 1-8 of 8
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Assessment of temporal and spatial evolution of bacterial communities in a biological sand filter mesocosm treating winery wastewater
Ramond, Jean-Baptiste; Welz, P.J.; Tuffin, Marla; Burton, Stephanie G. (The Society for Applied Microbiology, 2013)AIMS: To assess the impact of winery wastewater (WW) on biological sand filter (BSF) bacterial community structures, and to evaluate whether BSFs can constitute alternative and valuable treatment-processes to remediate WW. ... -
Biodiversity: so much more than legs and leaves
Cowan, Donald A.; Rybicki, Edward P.; Tuffin, Marla; Valverde, Angel; Wingfield, Michael (AOSIS OpenJournals, 2013)Microorganisms inhabit virtually every possible niche on Earth, including those at the outer envelope of survival. However, the focus of most conservation authorities and ecologists is the ‘legs and leaves’ side of biology ... -
Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids
Musingarimi, Wicleffe; Tuffin, Marla; Cowan, Donald A. (Academy of Science of South Africa, 2010)An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the ... -
Cloning, expression and characterization of thermostable YdaP from Bacillus licheniformis 9A
Lako, Joseph D. Wani; Yengkopiong, Jada P.; Stafford, William H. L.; Tuffin, Marla; Cowan, Don A. (Acta Biochimica Polonica, 2017)The Bacillus licheniformis ydaP gene encodes for a pyruvate oxidase that catalyses the oxidative decarboxylation of pyruvate to acetate and CO2. The YdaP form of this enzyme was purified about 48.6-folds to homogeneity in ... -
Engineering pyruvate decarboxylase-mediated ethanol production in the thermophilic host Geobacillus thermoglucosidasius
Van Zyl, L.J.; Taylor, M.P.; Eley, K.; Tuffin, Marla; Cowan, Donald A. (Springer Verlag, 2013)This study reports the expression, purification, and kinetic characterization of a pyruvate decarboxylase (PDC) from Gluconobacter oxydans . Kinetic analyses showed the enzyme to have high affinity for pyruvate (120 μM at ... -
Extremophiles in biofuel synthesis
Barnard, Desire; Casanueva, Ana; Tuffin, Marla (Taylor and Francis Group, 2010)The current global energy situation has demonstrated an urgent need for the development of alternative fuel sourcesto the continually diminishing fossil fuel reserves. Much research to address this issue focuses on the ... -
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils
Zablocki, Olivier; van Zyl, Lonnie; Adriaenssens, Evelien M.; Rubagotti, Enrico; Tuffin, Marla; Cary, Stephen Craig; Cowan, Donald A. (American Society for Microbiology, 2014)The metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities, cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded ... -
Micro-Eukaryotic diversity in Hypolithons from Miers Valley, Antarctica
Gokul, Jarishma K.; Valverde, Angel; Tuffin, Marla; Cary, Stephen Craig; Cowan, Donald A. (Multidisciplinary Digital Publishing Institute (MDPI), 2013)The discovery of extensive and complex hypolithic communities in both cold and hot deserts has raised many questions regarding their ecology, biodiversity and relevance in terms of regional productivity. However, most ...