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Inferring bona fide transfrags in RNA-Seq derived-transcriptome assemblies of non-model organisms
(BioMed Central, 2015)
Background: De novo transcriptome assembly of short transcribed fragments (transfrags) produced from sequencing-by-synthesis technologies often results in redundant datasets with differing levels of unassembled, partially ...
A glance at quality score: implication for de novo transcriptome reconstruction of Illumina reads
Downstream analyses of short-reads from next-generation sequencing platforms are often preceded by a pre-processing step that removes uncalled and wrongly called bases. Standard approaches rely on their associated base ...