| dc.contributor.author | Berry, Michael | |
| dc.contributor.author | Manasse, Taryn-Lee | |
| dc.contributor.author | Tan, Yee-Joo | |
| dc.contributor.author | Fielding, Burtram C. | |
| dc.date.accessioned | 2013-10-06T20:24:01Z | |
| dc.date.available | 2013-10-06T20:24:01Z | |
| dc.date.issued | 2012 | |
| dc.identifier.citation | Berry, M., et al. (2012). Characterisation of human coronavirus-NL63 nucleocapsid protein. African Journal of Biotechnology, 11(75): 13962-13968 | en_US |
| dc.identifier.issn | 1684–5315 | |
| dc.identifier.uri | http://hdl.handle.net/10566/735 | |
| dc.description.abstract | Coronavirus N is a multifunctional protein that plays an essential role in enhancing the efficiency of virus transcription and assembly. This manuscript reports the analysis of HCoV-NL63 N protein by comparing the amino acid sequences of coronavirus N-homologues. A ~50 kDa protein was expressed in both a mammalian cell and bacterial cell system that is similar in size to the predicted ~42.6 kDa HCoV-NL63 N protein. PSORTII identified two putative nuclear localisations signals and PONDR identified one disordered region in HCoV-NL63 N. The reported protein analysis serves as a prelude to laboratory analysis to understand the processing of HCoV-NL63 N. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Academic Journals | en_US |
| dc.rights | © 2012 Berry, et al; licensee Academic Journals. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
| dc.source.uri | http://dx.doi.org/10.5897/AJB12.1657 | |
| dc.subject | HCoV-NL63 | en_US |
| dc.subject | Nucleocapsid protein | |
| dc.subject | Protein analysis | |
| dc.subject | Nuclear localisation signals | |
| dc.subject | Protein ordered/disordered regions | |
| dc.title | Characterisation of human coronavirus-NL63 nucleocapsid protein | en_US |
| dc.type | Article | en_US |
| dc.privacy.showsubmitter | false | |
| dc.status.ispeerreviewed | true | |
| dc.description.accreditation | Web of Science | en_US |
