Musingarimi, Wicleffe; Tuffin, I. Marla; Cowan, Donald A.(Academy of Science of South Africa, 2010)
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Abstract:
An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD)
neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant
Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone
conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the
arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase),
arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known
arsenic resistance operons. The Bacillus sp. UWC arsRBCDA genes were shown to be arranged in
an unusual manner with the arsDA genes immediately downstream of arsC.