Sjoling, Sara; Cowan, Donald A.(Springer Verlag, 2003)
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Abstract:
The microbial diversity in maritime meltwater
pond sediments from Bratina Island, Ross Sea, Antarctica
was investigated by 16S rDNA-dependent
molecular phylogeny. Investigations of the vertical
distribution, phylogenetic composition, and spatial
variability of Bacteria and Archaea in the sediment were
carried out. Results revealed the presence of a highly
diverse bacterial population and a significantly depthrelated
composition. Assessment of 173 partial 16S
rDNA clones analyzed by amplified rDNA restriction
analysis (ARDRA) using tetrameric restriction enzymes
(HinP1I 5'GVCGC3'and Msp I. 5'CVGG3', BioLabs)
revealed 153 different bacterial OTUs (operational taxonomic
units). However, only seven archaeal OTUs
were detected, indicating low archaeal diversity. Based
on ARDRA results, 30 bacterial clones were selected for
sequencing and the sequenced clones fell into seven
major lineages of the domain Bacteria; the a, c, and d
subdivisions of Proteobacteria, the Cytophaga–Flavobacterium–
Bacteroides, the Spirochaetaceae, and the
Actinobacteria. All of the archaeal clones sequenced
belonged to the group Crenarchaeota and phylogenetic
analysis revealed close relationships with members of the
deep-branching Group 1 Marine Archaea.