Now showing items 17-24 of 24

  • Non-specificity of Staphylococcus generic primers 

    Ah Tow, Lemese; Cowan, Donald A. (Society for General Microbiology, 2003)
    Our results allow us to conclude that there appears to be significant conservation between the tuf genes of Planococcus, Planomicrobium and Staphylococcus spp., and that although the primer set TstaG422/TStag765 has ...
  • PCR-based detection of non-indigenous microorganisms in ‘pristine’ environments 

    Baker, Gillian; Ah Tow, Lemese; Cowan, Donald A. (Elsevier, 2003)
    PCR-based technologies are widely employed for the detection of specific microorganisms, and may be applied to the identification of non-indigenous microorganisms in ‘pristine’ environments. For ‘pristine’ environments ...
  • Purification, crystallization and preliminary X-ray diffraction analysis of thermostable nitrile hydratase: research letter 

    Cowan, Donald A.; Sayed, Muhammed F.; Tsekoa, Tsepo L.; Cameron, Rory A.; Sewell, B. Trevor (Academy of Science of South Africa (ASSAf), 2004)
    Microbial nitrile hydratases are important industrial enzymes that catalyse the conversion of nitriles to the corresponding amides. Bacillus strain RAPc8 nitrile hydratase has recently been cloned and functionally ...
  • Review and re-analysis of domain-specific 16S primers 

    Baker, Gillian; Smith, Jacques J.; Cowan, Donald A. (Elsevier, 2003)
    The Polymerase Chain Reaction (PCR) has facilitated the detection of unculturable microorganisms in virtually any environmental source and has thus been used extensively in the assessment of environmental microbial diversity. ...
  • The search for the ideal biocatalyst 

    Burton, Stephanie; Cowan, Donald A.; Woodley, John M. (Nature Publishing Group, 2002)
    While the use of enzymes as biocatalysts to assist in the industrial manufacture of fine chemicals and pharmaceuticals has enormous potential, application is frequently limited by evolution-led catalyst traits. The advent ...
  • Sequence analysis of an Archaeal virus isolated from a hypersaline lake in Inner Mongolia, China 

    Pagaling, Eulyn; Haigh, Richard D.; Grant, William D.; Cowan, Donald A.; Jones, Brian; Ma, Yanhe; Ventosa, Antonio; Heaphy, Shaun (BioMed Central, 2007)
    Background: We are profoundly ignorant about the diversity of viruses that infect the domain Archaea. Less than 100 have been identified and described and very few of these have had their genomic sequences determined. ...
  • Structure of an aliphatic amidase from Geobacillus pallidus RAPc8 

    Kimani, Serah W.; Agarkar, Vinod B.; Cowan, Donald A.; Sayed, Muhammed F.; Sewell, B. Trevor (International Union of Crystallography, 2007)
    The amidase from Geobacillus pallidus RAPc8, a moderate thermophile, is a member of the nitrilase superfamily and catalyzes the conversion of amides to the corresponding carboxylic acids and ammonia. It shows both ...
  • The upper temperature for life – where do we draw the line? 

    Cowan, Donald A. (Elsevier, 2004)
    The newly isolated hyperthermophilic archaeal strain 121 grows slowly at 121 8C and even survives short periods at 130 8C. This is another organism that grows best at temperatures well in excess of 100 8C! We should not ...