Browsing Institute for Microbial Biotechnology & Metagenomics by Author "Jaenicke, Thomas"
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Wilkinson, Dianne E.; Jaenicke, Thomas; Cowan, Donald A. (Kluwer Academic Publishers, 2002)[more][less]
Abstract: An efficient and simple method for constructing an environmental library using mechanically sheared DNA obtained directly from geothermal sediments is presented. The method is based on blunt-end modification of DNA fragments followed by 3' -adenylation using Vent DNA polymerase and Taq DNA polymerase, respectively. The prepared DNA fragments are then ligated into a TA cloning vector and used in the transformation of Escherichia coli. This method has been successfully applied to the cloning of ORFs derived from uncultivated prokaryotes present in geothermal sediment. URI: http://hdl.handle.net/10566/161 Files in this item: 1
WilkinsonEfficientMolecular2002.pdf (754.4Kb)
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