dc.contributor.author | Zablocki, Olivier | |
dc.contributor.author | van Zyl, Leonardo Joaquim | |
dc.contributor.author | Kirby, Bronwyn | |
dc.contributor.author | Trindade, Marla | |
dc.date.accessioned | 2017-12-13T10:09:15Z | |
dc.date.available | 2017-12-13T10:09:15Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Zablocki, O. et al. (2017). Diversity of dsDNA viruses in a South African hot spring assessed by metagenomics and microscopy. Viruses, 9: 384 | en_US |
dc.identifier.issn | 1999-4915 | |
dc.identifier.uri | http://dx.doi.org/10.3390/v9110348 | |
dc.identifier.uri | http://hdl.handle.net/10566/3328 | |
dc.description.abstract | The current view of virus diversity in terrestrial hot springs is limited to a few sampling
sites. To expand our current understanding of hot spring viral community diversity, this study
aimed to investigate the first African hot spring (Brandvlei hot spring; 60 C, pH 5.7) by means
of electron microscopy and sequencing of the virus fraction. Microscopy analysis revealed a
mixture of regular- and ‘jumbo’-sized tailed morphotypes (Caudovirales), lemon-shaped virions
(Fuselloviridae-like; salterprovirus-like) and pleiomorphic virus-like particles. Metavirome analysis
corroborated the presence of His1-like viruses and has expanded the current clade of salterproviruses
using a polymerase B gene phylogeny. The most represented viral contig was to a cyanophage genome
fragment, which may underline basic ecosystem functioning provided by these viruses. Furthermore,
a putative Gemmata-related phage was assembled with high coverage, a previously undocumented
phage-host association. This study demonstrated that a moderately thermophilic spring environment
contained a highly novel pool of viruses and should encourage future characterization of a wider
temperature range of hot springs throughout the world. | en_US |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |
dc.subject | Hot spring | en_US |
dc.subject | Metavirome | en_US |
dc.subject | Fuselloviridae | en_US |
dc.subject | Jumbo phage | en_US |
dc.subject | Archaeal viruses | en_US |
dc.subject | Freshwater Cyanophages | en_US |
dc.subject | Gemmata | en_US |
dc.title | Diversity of dsDNA viruses in a South African hot spring assessed by metagenomics and microscopy | en_US |
dc.type | Article | en_US |
dc.privacy.showsubmitter | FALSE | |
dc.status.ispeerreviewed | TRUE | |