Browsing Research Articles (Medical Bioscience) by Author "Agarwal, A."
Now showing items 1-7 of 7
-
Association between promoter methylation of MLH1 and MSH2 and reactive oxygen species in oligozoospermic men—A pilot study
Gunes, S.; Agarwal, A.; Henkel, Ralf; Sharma, Rajan; Esteves, S.C.; Aljowair, A.; Emirzeoglu, D.; Alkhani, A.; Pelegrini, L.; Joumah, A.; Sabanegh, E.; Mahmutoglu, A.M. (Wiley, 2017)MLH1 and MSH2 are important genes for DNA mismatch repair and crossing over during meiosis and are implicated in male infertility. Therefore, the methylation patterns of the DNA mismatch repair genes MLH1 and MSH2 in ... -
Calibration of redox potential in sperm wash media and evaluation of oxidation–reduction potential values in various assisted reproductive technology culture media using MiOXSYS system
Panner Selvam, M. K.; Henkel, Ralf; Sharma, Rajan; Agarwal, A. (Wiley, 2018)Oxidation–reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to ... -
Causes and consequences of sperm mitochondrial dysfunction
Durairajanayagam, D.; Singh, D.; Agarwal, A.; Henkel, R. (Wiley-Blackwell, 2020)Mitochondria have multiple functions, including synthesis of adenine triphosphate, production of reactive oxygen species, calcium signalling, thermogenesis and apoptosis. Mitochondria have a significant contribution in ... -
Determination of seminal oxidation–reduction potential (ORP) as an easy and cost-effective clinical marker of male infertility
Agarwal, A.; Henkel, Ralf; Sharma, Rajan; Tadros, N.N.; Sabanegh, E. (Wiley, 2017)Oxidative stress (OS) is an important contributing factor to male infertility. While previous methods to measure seminal OS are time-consuming and limited to the use of freshly produced semen, oxidation reduction potential ... -
Evaluation of reference values of standard semen parameters in fertile Egyptian men
Zedan, H.; Ismail, S.; Gomaa, A.; Saleh, R.; Henkel, Ralf; Agarwal, A. (Wiley, 2018)The reference values of human semen, published in the WHO’s latest edition in 2010, were lower than those previously reported. The objective of this study was to evaluate reference values of standard semen parameters in ... -
Human sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation–reduction potential
Roychoudhury, S.; Maldonado-Rosas, I.; Agarwal, A.; Esteves, S.C.; Henkel, Ralf; Sharma, Rajan (Wiley, 2018)Polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) are routinely used in handling spermatozoa for intracytoplasmic sperm injection (ICSI). As there are still concerns about possible adverse effects on the embryo, this ... -
In Silico Sperm Proteome Analysis to Investigate DNA Repair Mechanisms in Varicocele Patients
Henkel, Ralf; Finelli, R; Darbandi, S; Pushparaj, P. N.; Ko, E.; Agarwal, A. (Frontiers Media S.A., 2021-12-17)Varicocele, a condition associated with increased oxidative stress, negatively affects sperm DNA integrity and reduces pregnancy rates. However, the molecular mechanisms related to DNA integrity, damage, and repair in ...