dc.contributor.author | Vij, Shubha | |
dc.contributor.author | Van Heusden, Peter | |
dc.contributor.author | Christoffels, Alan | |
dc.contributor.author | Mbandi, Stanley K. | |
dc.contributor.author | Mwangi, Sarah | |
dc.date.accessioned | 2016-04-24T17:53:24Z | |
dc.date.available | 2016-04-24T17:53:24Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Vij, S. et al. (2016). Chromosomal-level assembly of the Asian seabass genome using long sequence reads and multi-layered scaffolding. PLoS Genetics, 12(4): e1005954 | en_US |
dc.identifier.issn | 1553-7404 | |
dc.identifier.uri | http://hdl.handle.net/10566/2133 | |
dc.description.abstract | We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a
tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical
and genetic mapping along with shared synteny from closely related fish species to
derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50
size over 25 Mb that span ~90% of the genome. The population structure of L. calcarifer species complex was analyzed by re-sequencing 61 individuals representing various
regions across the species’ native range. SNP analyses identified high levels of genetic
diversity and confirmed earlier indications of a population stratification comprising three
clades with signs of admixture apparent in the South-East Asian population. The quality of
the Asian seabass genome assembly far exceeds that of any other fish species, and will
serve as a new standard for fish genomics. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | This is an open access article, free of all
copyright, and may be freely reproduced, distributed,
transmitted, modified, built upon, or otherwise used
by anyone for any lawful purpose. The work is made
available under the Creative Commons CC0 public
domain dedication. | |
dc.source.uri | http://dx.doi.org/10.1371/journal.pgen.1005954 | |
dc.subject | Asian seabass | en_US |
dc.subject | Lates calcarifer | en_US |
dc.subject | Chromosomes | en_US |
dc.subject | Fish species | en_US |
dc.subject | DNA sequencing | en_US |
dc.subject | Genome sequencing | en_US |
dc.subject | North-Western India | en_US |
dc.subject | South-East Asia | en_US |
dc.subject | Australia | en_US |
dc.subject | Papua New Guinea | en_US |
dc.title | Chromosomal-level assembly of the Asian seabass genome using long sequence reads and multi-layered scaffolding | en_US |
dc.type | Article | en_US |
dc.privacy.showsubmitter | FALSE | |
dc.status.ispeerreviewed | TRUE | |
dc.description.accreditation | Web of Science | en_US |