Gene expression modulation of apoptotic and oestrogen receptor alpha genes by active fractions of selected Nigerian plants on cervical cancer cell line (hela)
Date
2021Author
Morounke, Saibu G.
Ayorinde, James B.
Olawale, Fadaka O.
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Show full item recordAbstract
The roles of natural product in drug discovery and development cannot be over emphasised. It
plays a vital role in human therapy and gives a better understanding on the cellular
pathways.This study investigated the modulatory effects of partially purified fractions of Piper
guineense Schumach. & Thonn. (Piperaceae), Zanthoxylum zanthoxyloides Lam. (Rutaceae),
Amaranthus viridis L. (Amaranthaceae), Costus afer Ker-Gawl. (Zingiberaceae) and
Catharanthus roseus (L.) G. Don. (Apocynaceae) on oestrogen receptor-α (ESR-α), tumour
protein p53 (TP-53), retinoblastoma (RB) and NAD(P) H quinine oxidoreductase (NQO1)
genes in cervical cancer cell line (HeLa cells). n-Hexane, ethylacetate, chloroform, and water
fractions of 80% ethanol extracts of study plants were screened with brine shrimp lethality and
water-soluble tetrazolium-1 (WST-1) cytotoxicity assay. HeLa cells were treated with 1:10
dilutions of IC50 concentrations of test fractions for 24 hours, total RNA was extracted, RNA
quality was checked, and normalized to a baseline concentration. Gene expression were
monitored by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
Results showed that ESR-α was downregulated (p < 0.05) by P. guineense-hexane, C. roseuschloroform and A. viridis-ethylacetate fractions.