Browsing Faculty of Natural Sciences by Issue Date
Now showing items 41-60 of 2571
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The contribution of exon-skipping events on chromosome 22 to protein coding diversity
(Cold Spring Harbor Laboratory Press, 2001)Completion of the human genome sequence provides evidence for a gene count with lower bound 30,000–40,000. Significant protein complexity may derive in part from multiple transcript isoforms. Recent EST based studies have ... -
A hydrogeomorphological approach to quantification of groundwater discharge to streams in South Africa
(Water Research Commission, 2002)In South Africa, the flow requirements for maintaining the normal functioning of aquatic ecosystems is termed the “ecological reserve”, and these should be determined when a licence application for water allocation is ... -
Capture zone simulation for boreholes located in fractured dykes using the linesink concept
(Water Research Commission, 2002)Delineation of capture zones for groundwater source protection is normally performed by using numerical codes which are based on the porous medium flow equation. However, boreholes are often sited in or along permeable ... -
Efficient molecular cloning of environmental DNA from geothermal sediments
(Kluwer Academic Publishers, 2002)An efficient and simple method for constructing an environmental library using mechanically sheared DNA obtained directly from geothermal sediments is presented. The method is based on blunt-end modification of DNA fragments ... -
The search for the ideal biocatalyst
(Nature Publishing Group, 2002)While the use of enzymes as biocatalysts to assist in the industrial manufacture of fine chemicals and pharmaceuticals has enormous potential, application is frequently limited by evolution-led catalyst traits. The advent ... -
SoftBridge: an architecture for building IP-based bridges over the digital divide
(Telkom, 2002)Users and computing equipment have varying capabilities that include the abilities to exchange text and audio, engage in high bandwidth communication, etc. As the technological options increase, e ective communication ... -
Telgo323: an H.323 Bridge for deaf telephony
(Telkom, 2002)We have developed a prototype bridge that relays text and speech between Teldem, a text telephone for the Deaf, and a standard telephone or H.323 endpoint. Telgo323 uses modified H.323 media gateways and open source Text ... -
Antarctic Dry Valley mineral soils contain unexpectedly high levels of microbial biomass
(Springer Verlag, 2002)We have applied bioluminescent ATP detection methods to microbial enumeration in Antarctic Dry Valley mineral soils, and validated our ATP data by two independent methods. We have demonstrated that ATP measurement is ... -
Biodegradation of high-concentration isopropanol by a solvent-tolerant thermophile, Bacillus pallidus
(Springer Verlag, 2002)The aerobic biodegradation of high-concentration, to 24 g l –1 , 2-propanol (IPA) by a thermophilic isolate ST3, identified as Bacillus pallidus , was successfully carried out for the first time. This solvent-tolerant B. ... -
The genetic organisation of a 2,966 basepair DNA fragment of a Single Capsid Nucleopolyhedrovirus isolated from Trichoplusia ni
(Springer Verlag, 2002)In order to investiagte the genomic organization of the Trichoplusia ni Single Capsid Nucleoplyhedrovirus (TnSNPV) , a 2,966 base pairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading ... -
Development of infectious transcripts and genome manipulation of black queen-cell virus of honey bees
(Microbiology Society, 2002)The South African isolate of Black queen-cell virus (BQCV), a honey bee virus, was previously found to have an 8550 nucleotide genome excluding the poly(A) tail. Its genome contained two ORFs, a 5«-proximal ORF encoding ... -
Development of molecular tools for honeybee virus research: the South African contribution
(Academic Journals, 2003)Increasing knowledge of the association of honeybee viruses with other honeybee parasites, primarily the ectoparasitic mite Varroa destructor, and their implication in the mass mortality of honeybee colonies has resulted ... -
High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica
(Springer Verlag, 2003)The microbial diversity in maritime meltwater pond sediments from Bratina Island, Ross Sea, Antarctica was investigated by 16S rDNA-dependent molecular phylogeny. Investigations of the vertical distribution, phylogenetic ... -
Using voice over IP to bridge the digital divide: a critical action research approach
(Telkom, 2003)There is a great disparity between those who have access to Information and Communication Technologies (ICTs) and those that do not. This phenomenon forms part of the Digital Divide. Many ICTs may be used to help overcome ... -
SoftBridge in action: the first Deaf telephony pilot
(Telkom, 2003)Following on several partial prototypes, we built an automated Deaf Telephony bridging application with the SoftBridge platform. The SoftBridge performs multi-modal bridging in real-time using Text-to-Speech (TTS) and ... -
Observations on the ecology of Pleurobrachia pileus (Ctenophora) in the southern Benguela ecosystem
(NISC (Pty) Ltd and Taylor & Francis, 2003)The vertical distribution, abundance and size composition of a population of Pleurobrachia pileus was studied during a six-day drogue study conducted off the west coast of South Africa in February 1995. The population was ... -
Non-specificity of Staphylococcus generic primers
(Society for General Microbiology, 2003)Our results allow us to conclude that there appears to be significant conservation between the tuf genes of Planococcus, Planomicrobium and Staphylococcus spp., and that although the primer set TstaG422/TStag765 has ... -
Floor control arbitration for a hybrid voice/text web-board
(Telkom, 2003)We have built a hybrid voice/text web–board that utilizes floor control for handling resources such as microphone and interface events. The aim of this research is to determine the most appropriate floor control mechanism ... -
SoftBridge: a multimodal instant messaging bridging system
(Telkom, 2003)Instant Messaging is traditionally a text only a air. However, there are instances when it would be useful to bridge to other types of media, like speech. The SoftBridge is an application framework that enables this kind ... -
Provision of syndromic treatment of sexually transmitted infections by community pharmacists: a potentially underutilized HIV prevention strategy
(Lippincott, Williams & Wilkins, 2003)Background: Sexually transmitted infections (STIs) are known risk factors for HIV infection. Goal: The goal of this study was to assess the current and potential future role that community pharmacists in Western Cape, South ...