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dc.contributor.authorSjoling, Sara
dc.contributor.authorCowan, Donald A.
dc.date.accessioned2010-09-23T14:57:54Z
dc.date.available2010-09-23T14:57:54Z
dc.date.issued2003
dc.identifier.citationSjoling, S. & Cowan, D.A. (2003). High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica. Extremophiles, 7:275-282.en_US
dc.identifier.urihttp://hdl.handle.net/10566/148
dc.description.abstractThe microbial diversity in maritime meltwater pond sediments from Bratina Island, Ross Sea, Antarctica was investigated by 16S rDNA-dependent molecular phylogeny. Investigations of the vertical distribution, phylogenetic composition, and spatial variability of Bacteria and Archaea in the sediment were carried out. Results revealed the presence of a highly diverse bacterial population and a significantly depthrelated composition. Assessment of 173 partial 16S rDNA clones analyzed by amplified rDNA restriction analysis (ARDRA) using tetrameric restriction enzymes (HinP1I 5'GVCGC3'and Msp I. 5'CVGG3', BioLabs) revealed 153 different bacterial OTUs (operational taxonomic units). However, only seven archaeal OTUs were detected, indicating low archaeal diversity. Based on ARDRA results, 30 bacterial clones were selected for sequencing and the sequenced clones fell into seven major lineages of the domain Bacteria; the a, c, and d subdivisions of Proteobacteria, the Cytophaga–Flavobacterium– Bacteroides, the Spirochaetaceae, and the Actinobacteria. All of the archaeal clones sequenced belonged to the group Crenarchaeota and phylogenetic analysis revealed close relationships with members of the deep-branching Group 1 Marine Archaea.en_US
dc.language.isoenen_US
dc.publisherSpringer Verlagen_US
dc.rightsThis is the author post-print version of an article published by Springer
dc.source.urihttp://dx.doi.org/10.1007/s00792-003-0321-z
dc.subjectSedimenten_US
dc.subject16S rDNAen_US
dc.subjectAntarcticaen_US
dc.subjectArchaeaen_US
dc.subjectARDRA (amplified rDNA restriction analysis)en_US
dc.subjectMicrobial diversityen_US
dc.subjectEnvironmental bacteriaen_US
dc.titleHigh 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarcticaen_US
dc.typeArticleen_US
dc.privacy.showsubmitterfalse
dc.status.ispeerreviewedtrue


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