Browsing Research Articles (Biotechnology) by Author "Klein, Ashwil"
Now showing items 1-15 of 15
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Capacity to control oxidative stress-induced caspase-like activity determines the level of tolerance to salt stress in two contrasting maize genotypes
Keyster, Marshall; Klein, Ashwil; Du Plessis, Morné; Jacobs, Alex; Kappo, Abidemi; Kocsy, Gábor; Galiba, Gábor; Ludidi, Ndiko (Springer, 2013)The response of two maize (Zea mays L.) genotypes, named GR (salt-tolerant) and SK (salt-sensitive), to salt stress (150 mM NaCl) was investigated under controlled environmental growth conditions. Genotype SK experienced ... -
Comparative venom toxin analyses of Nigerian viperidae and elapidae snakes
Adamude, Fatima Amin; Dingwoke, Emeka John; Klein, Ashwil (Elsevier, 2023)Envenoming by snakebite is a serious health problem that maims and kills a large number of people, primarily in rural areas of developing African countries. The first comparative venom proteomic analyses of four snakes ... -
Development of Effective Therapeutic Molecule from Natural Sources against Coronavirus Protease
Fadaka, Adewale Oluwaseun; Sibuyi, Nicole Remaliah Samantha; Martin, Darius Riziki; Klein, Ashwil (MDPI, 2021)Abstract: The SARS-CoV-2 main protease (Mpro) is one of the molecular targets for drug design. Effective vaccines have been identified as a long-term solution but the rate at which they are being administered is slow in ... -
Diagnostic approaches of pneumonia for commercial-scale biomedical applications: An overview
Bakare, Olalekan Olanrewaju; Fadaka, Adewale Oluwaseun; Klein, Ashwil (Taylor & Francis, 2020)Pneumonia remains the leading infectious cause of death among children under five years of age, and the elderly. Several biomarkers, which have been identified for its diagnosis lack specificity, as they could not ... -
Dietary effects of antimicrobial peptides in therapeutics
Bakare, Olalekan Olanrewaju; Fadaka, Adewale Oluwaseun; Klein, Ashwil; Pretorius, Ashley (Taylor & Francis, 2020-02-17)The notable increase in drug-resistant infections and the failure of the most potent antibiotics to establish their curative effect without side effect have presented a serious need for the discovery of new therapeutic ... -
Drought and salinity stress alters ROS accumulation, water retention, and osmolyte content in sorghum plants
Nxele, Xolisa; Klein, Ashwil; Ndimba, Bongani Kaiser (Elsevier, 2017)biotic stresses such as drought and salinity are major environmental factors that influence crop productivity worldwide. These adverse conditions induce osmotic stresses in plant cells by decreasing water availability, thus ... -
Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea (causative pathogen of gray mold)
Kgang, Itumeleng E.; Klein, Ashwil; Mohamed, Gadija G. (Frontiers Media, 2023)Essential oils (EOs) have been demonstrated as efficacious against B. cinerea. However, the underpinning enzymatic and proteomic mechanism for these inhibitory effects is not entirely clear. Thus, this study examined the ... -
Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against botrytis cinerea (causative pathogen of gray mold)
Klein, Ashwil; Kgang, Itumeleng Eugenia; Mohamed, Gadija G.; Mathabe, Patricia M. K.; Belay, Zinash A; Caleb, Oluwafemi James (Front. Microbiol, 2023)Introduction: Essential oils (EOs) have been demonstrated as efficacious against B. cinerea. However, the underpinning enzymatic and proteomic mechanism for these inhibitory effects is not entirely clear. Methods: Thus, ... -
Exogenous p-coumaric acid improves salvia hispanica l. Seedling shoot growth
Nkomo, Mbukeni; Gokul, Arun; Keyster, Marshall; Klein, Ashwil (MDPI Open Access Journals, 2019)p-Coumaric acid (p-CA) belongs to a family of natural esters of hydroxycinnamic acid compounds that have been shown to modulate plant growth and metabolism. In this study, we investigated the effect of exogenous p-CA on ... -
Functional prediction of candidate micrornas for CRC management using in silico approach
Fadaka, Adewale Oluwaseun; Pretorius, Ashley; Klein, Ashwil (MDPI, 2019)Approximately 30–50% of malignant growths can be prevented by avoiding risk factors and implementing evidence-based strategies. Colorectal cancer (CRC) accounted for the second most common cancer and the third most common ... -
Modulation of antioxidant enzyme activities and metabolites ratios by nitric oxide in short-term salt stressed soybean root nodules
Egbichi, I.; Keyster, Marshall; Jacobs, Alex; Klein, Ashwil; Ludidi, Ndiko (Elsevier, 2013)Several abiotic factors cause molecular damage to plants either directly or through the accumulation of reactive oxygen species such as hydrogen peroxide (H2O2). We investigated if application of nitric oxide (NO) donor ... -
Nitric oxide (NO) regulates the expression of single-domain cystatins in glycine max (soybean)
Keyster, Marshall; Adams, Ruqaiyah; Klein, Ashwil; Ludidi, Ndiko (Southern Cross Publishing, 2013)Plant cystatins inhibit cysteine proteases and are important in regulating plant development and plant responses to biotic and abiotic stress. Furthermore, nitric oxide plays a signaling role in regulating plant responses ... -
Response of soybean nodules to exogenously applied caffeic acid during NaCl-induced salinity
Klein, Ashwil; Keyster, Marshall; Ludidi, Ndiko N. (Elsevier, 2015)affeic acid acts as an antioxidant to scavenge reactive oxygen species, but its influence on plant responses to abi- otic stresses is only partially understood. Here, we investigated the influence of exogenously applied ... -
Sustainable agriculture through the enhancement of microbial biocontrol agents: Current challenges and new perspectives
Gokul, Arun; Mabaso, Jabulani; Klein, Ashwil (MDPI, 2023)The future of pesticide usage in agriculture is uncertain due to its unsustainability, adverse environmental impacts, and its association in enhanced phytopathogen resistance. Hence, this situation urges the development ... -
Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species
Dingwoke, Emeka John; Adamude, Fatima Amin; Klein, Ashwil (Elsevier, 2021)Snakebite envenoming remains a neglected tropical disease which poses severe health hazard, especially for the rural inhabitants in Africa. In Nigeria, vipers are responsible for the highest number of deaths. Hydrophili ...