| dc.description.abstract | The blood-brain barrier (BBB) is a central nervous system protective barrier
formed primarily of endothelial cells that regulate the entry of substances and cells from entering the
brain. However, the BBB integrity is disrupted in disease, including cancer, allowing toxic substances,
molecules, and circulating cells to enter the brain. This study aimed to determine the mitochondrial
changes in brain endothelial cells co-cultured with cancer cells. Method: Brain endothelial cells
(bEnd.3) were co-cultivated with various concentrations of breast cancer (MCF7) conditioned media
(CM) generated under normoxic (21% O2
) and hypoxic conditions (5% O2
). The mitochondrial
activities (including; dehydrogenases activity, mitochondrial membrane potential (∆Ψm), and ATP
generation) were measured using Polarstar Omega B.M.G-Plate reader. Trans-endothelial electrical
resistance (TEER) was evaluated using the EVOM system, followed by quantifying gene expression
of the endothelial tight junction (ETJs) using qPCR. Results: bEnd.3 cells had reduced cell viability
after 72 h and 96 h exposure to MCF7CM under hypoxic and normoxic conditions. | en_US |
