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Chromosomal-level assembly of the Asian seabass genome using long sequence reads and multi-layered scaffolding
(Public Library of Science, 2016)
We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a
tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical
and genetic mapping along with shared ...
Inferring bona fide transfrags in RNA-Seq derived-transcriptome assemblies of non-model organisms
(BioMed Central, 2015)
Background: De novo transcriptome assembly of short transcribed fragments (transfrags) produced from sequencing-by-synthesis technologies often results in redundant datasets with differing levels of unassembled, partially ...
A glance at quality score: implication for de novo transcriptome reconstruction of Illumina reads
(Frontiers, 2014)
Downstream analyses of short-reads from next-generation sequencing platforms are often preceded by a pre-processing step that removes uncalled and wrongly called bases. Standard approaches rely on their associated base ...