Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT
Date
2006Author
Fielding, Burtram C.
Gunalan, Vithiagaran
Tan, Timothy H.P.
Chou, Chih-Fong
Shen, Shuo
Khan, Sehaam
Lim, Seng Gee
Hong, Wanjin
Tan, Yee-Joo
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Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we
report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and
human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular
co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide
repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane)
and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled
with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly
assembly of SARS-CoV.
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