Show simple item record

dc.contributor.authorMiller, William G.
dc.contributor.authorLastovica, Albert J.
dc.date.accessioned2016-06-08T12:17:00Z
dc.date.available2016-06-08T12:17:00Z
dc.date.issued2012
dc.identifier.citationMiller, W.G. et al. (2012). Multilocus sequence typing methods for the emerging Campylobacter species C. hyointestinalis, C. lanienae, C. sputorum, C. concisus, and C. curvus. Frontiers in Cellular and Infection Microbiology, 2: Article 45en_US
dc.identifier.issn2235-2988
dc.identifier.urihttp://hdl.handle.net/10566/2299
dc.description.abstractMultilocus sequence typing (MLST) systems have been reported previously for multiple food - and food animal-associated Campylobacter species (e.g., C. jejuni, C. coli, C. lari, and C. fetus) to both differentiate strains and identify clonal lineages. These MLST methods focused primarily on campylobacters of human clinical (e.g., C. jejuni) or veterinary (e.g., C. fetus) relevance. However, other, emerging, Campylobacter species have been isolated increasingly from environmental, food animal, or human clinical samples. We describe herein four MLST methods for five emerging Campylobacter species: C. hyointestinalis, C. lanienae, C. sputorum, C. concisus, and C. curvus. The concisus/curvus method uses the loci aspA, atpA, glnA, gltA, glyA, ilvD, and pgm, whereas the other methods use the seven loci defined for C. jejuni (i.e., aspA, atpA, glnA, gltA, glyA, pgm, and tkt). Multiple food animal and human clinical C. hyointestinalis (n = 48), C. lanienae (n = 34), and C. sputorum (n = 24) isolates were typed, along with 86 human clinical C. concisus and C. curvus isolates. A large number of sequence types were identified using all four MLST methods. Additionally, these methods speciated unequivocally isolates that had been typed ambiguously using other molecular-based speciation methods, such as 16S rDNA sequencing. Finally, the design of degenerate primer pairs for some methods permitted the typing of related species; for example, the C. hyointestinalis primer pairs could be used to type C. fetus strains. Therefore, these novel Campylobacter MLST methods will prove useful in differentiating strains of multiple, emerging Campylobacter species.en_US
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rights.uriFrontiers provides online free and open access to all of its research publications.
dc.source.urihttp://dx.doi.org/10.3389/fcimb.2012.00045
dc.subjectCampylobacter sputorumen_US
dc.subjectCampylobacteren_US
dc.subjectFood animalen_US
dc.subjectCampylobacter concisusen_US
dc.subjectBacteriaen_US
dc.titleMultilocus sequence typing methods for the emerging Campylobacter species C. hyointestinalis, C. lanienae, C. sputorum, C. concisus, and C. curvusen_US
dc.typeArticleen_US
dc.privacy.showsubmitterFALSE
dc.status.ispeerreviewedTRUE
dc.description.accreditationISIen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record