Reduced glucose tolerance and skeletal muscle GLUT4 and IRS1 content in cyclists habituated to a long-term low-carbohydrate, high-fat diet
Date
2020Author
Webster, Christopher C
van Boom, Kathryn Merle
Armin, Nur
Metadata
Show full item recordAbstract
Very little is known about how long-term (>6 months) adaptation to a low-carbohydrate, high-fat (LCHF) diet affects insulin
signaling in healthy, well-trained individuals. This study compared glucose tolerance; skeletal muscle glucose transporter 4
(GLUT4) and insulin receptor substrate 1 (IRS1) content; and muscle enzyme activities representative of the main energy
pathways (3-hydroxyacetyl-CoA dehydrogenase, creatine kinase, citrate synthase, lactate dehydrogenase, phosphofructokinase,
phosphorylase) in trained cyclists who followed either a long-term LCHF or a mixed-macronutrient (Mixed) diet. On separate
days, a 2-hr oral glucose tolerance test was conducted, and muscle samples were obtained from the vastus lateralis of fasted
participants.