The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome

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2021Author
Khalafalla, Kareim
Majzoub, Ahmad
Henkel, Ralf
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Our study objective was to assess the effect of various sperm DNA fragmentation
levels on clinical intracytoplasmic sperm injection outcome. This retrospective study
included 392 patients who underwent ICSI and performed sperm DNA fragmentation testing before the procedure. Based on sperm DNA fragmentation cut-off values, the patients were differentiated into 3 groups as <20%, 20%–30% and >30%.
According to the female status, patients were differentiated into favourable group
(n = 259) with female age <35 years and anti-Mullerian hormone level ≥7.1 pmol/L;
and unfavourable group (n = 133) with female age ≥35 years and anti-Mullerian hormone level ≤7.1 pmol/L. The patient's medical records were reviewed, and patient's
demographic, laboratory data including semen analysis, sperm DNA fragmentation
determined by means of sperm chromatin dispersion, hormonal profile and data regarding intracytoplasmic sperm injection cycle were collected.
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