Seminal plasma metabolomics profiles following long (4–7 days) and short (2 h) sexual abstinence periods

Abstract

Metabolomic profiling of seminal plasma has been suggested as a possible approach for a fast and non-invasive male infertility evaluation diagnosis. However, metabolomics profiles in normo-zoo spermic men have not been thoroughly investigated, and the influence of ejaculation-abstinence has not been described. To provide interim reference values and find associations between the metabolomics profiles of human seminal plasma and length of ejaculation-abstinence period in normozoospermic men. Semen samples collected after long (4–7 days) and short abstinence (2 h) from 31 normo-zoospermic males were assessed for routine quality parameters before the seminal plasma was separatedby centrifugation. Metabolomics profiles of the seminal plasma were then determined using un targeted Nuclear Magnetic Resonance Spectroscopy.