Browsing Research Articles (SANBI) by Author "Mbandi, Stanley K."
Now showing items 1-3 of 3
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Chromosomal-level assembly of the Asian seabass genome using long sequence reads and multi-layered scaffolding
Vij, Shubha; Van Heusden, Peter; Christoffels, Alan; Mbandi, Stanley K.; Mwangi, Sarah (Public Library of Science, 2016)We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared ... -
A glance at quality score: implication for de novo transcriptome reconstruction of Illumina reads
Mbandi, Stanley K.; Hesse, Uljana; Rees, Jasper G.; Christoffels, Alan (Frontiers, 2014)Downstream analyses of short-reads from next-generation sequencing platforms are often preceded by a pre-processing step that removes uncalled and wrongly called bases. Standard approaches rely on their associated base ... -
Inferring bona fide transfrags in RNA-Seq derived-transcriptome assemblies of non-model organisms
Mbandi, Stanley K.; Hesse, Uljana; Van Heusden, Peter; Christoffels, Alan (BioMed Central, 2015)Background: De novo transcriptome assembly of short transcribed fragments (transfrags) produced from sequencing-by-synthesis technologies often results in redundant datasets with differing levels of unassembled, partially ...