Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa
Date
2019Author
Iranpour, Farhad Golshan
Nateghian, Zohre
Henkel, Ralf
Metadata
Show full item recordAbstract
The aim of this study was to evaluate motility, viability, DNA integrity and apoptosis of
spermatozoa when washed semen samples were kept for up to 12 days at 4–6°C and
25°C. In this experimental study, 26 normozoospermic semen samples were washed
twice in Modified Ham's F10 and resuspended in IVF fertilisation medium. Half of the
specimens were stored at 4–6°C, and the other half was kept at 25°C for 12 days. The
proportions of viable, motile, spermatozoa with double-stranded DNA and apoptotic
spermatozoa were examined during storage time. Apoptosis was measured using annexin
V-PI staining followed by flow cytometry. Results showed that sperm motility
and viability decreased during 12 days of sample storage (p < .001). There was no
significant difference between the two temperatures in terms of motility and viability
for up to 2 days (p < .05).