Strengths and limitations of different Chromogenic Media for the identification of Candida species

Abstract

The treatment of invasive candidiasis and other Candida infections with the appropriate antifungal agent is assisted by the identification of Candida isolates to the species level. Rapid and accurate methods of differentiation are therefore imperative if treatment is to be effective, particularly in HIV-positive patients and in pregnant mothers where intervention may be necessary to reduce the risk for preterm delivery. The time used for isolation, identification and detection of mixed cultures may be reduced with the help of available chromogenic media. In this study, five commercial chromogenic media were evaluated for the differentiation of Candida species. Six type-strains of Candida species were streaked onto each of five different chromogenic media and incubated for up to 4 days at the different temperatures recommended by the manufacturers. This comparative evaluation demonstrated the strengths and weaknesses of each medium employed and found CHROMagar™ Candida and Chromogenic Candida Agar to be the most effective for distinguishing between different Candida species.