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Now showing items 11-20 of 23
16 S rDNA primers and the unbiased assessment of thermophile diversity
(Portland Press, 2004)
Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA.
‘Universal’ and domain-specific rRNA gene PCR primers have historically been used for the assessment
of microbial diversity ...
Metagenomics, gene discovery and the ideal biocatalyst
(Portland Press, 2004)
With the rapid development of powerful protein evolution and enzyme-screening technologies, there is
a growing belief that optimum conditions for biotransformation processes can be established without
the constraints of ...
Hypolithic and soil microbial community assembly along an aridity gradient in the Namib Desert
(Springer, 2013)
The Namib Dessert is considered the oldest desert in the world and hyperarid for the last 5 million years. However, the environmental buffering provided by quartz and other translucent rocks supports extensive hypolithic ...
Selection of diazotrophic bacterial communities in biological sand filter mesocosms used for the treatment of phenolic-laden wastewater
(Springer Verlag, 2013)
Agri effluents such as winery or olive mill waste-waters are characterized by high phenolic concentrations. These compounds are highly toxic and generally refractory to biodegradation. Biological sand filters (BSFs) represent ...
Engineering pyruvate decarboxylase-mediated ethanol production in the thermophilic host Geobacillus thermoglucosidasius
(Springer Verlag, 2013)
This study reports the expression, purification, and kinetic characterization of a pyruvate decarboxylase (PDC) from Gluconobacter oxydans . Kinetic analyses showed the enzyme to have high affinity for pyruvate (120 μM at ...
Review and re-analysis of domain-specific 16S primers
(Elsevier, 2003)
The Polymerase Chain Reaction (PCR) has facilitated the detection of unculturable microorganisms in virtually any environmental source and has thus been used extensively in the assessment of environmental microbial diversity. ...
Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids
(Academy of Science of South Africa, 2010)
An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD)
neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant
Escherichia coli strain for the ...
Antarctic Dry Valley mineral soils contain unexpectedly high levels of microbial biomass
(Springer Verlag, 2002)
We have applied bioluminescent ATP detection
methods to microbial enumeration in Antarctic Dry Valley
mineral soils, and validated our ATP data by two independent
methods. We have demonstrated that ATP measurement
is ...
Structure of an aliphatic amidase from Geobacillus pallidus RAPc8
(International Union of Crystallography, 2007)
The amidase from Geobacillus pallidus RAPc8, a moderate
thermophile, is a member of the nitrilase superfamily and
catalyzes the conversion of amides to the corresponding
carboxylic acids and ammonia. It shows both ...
PCR-based detection of non-indigenous microorganisms in ‘pristine’ environments
(Elsevier, 2003)
PCR-based technologies are widely employed for the detection of specific microorganisms, and may be applied to the identification of non-indigenous microorganisms in ‘pristine’ environments. For ‘pristine’ environments ...