Browsing by Author "den Haan, Riaan"
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Adapting the yeast consolidated bioprocessing paradigm for biorefineries
den Haan, Riaan (Green Wave Publishing of Canada, 2018)Despite decades long development, no natural or engineered organism has been isolated that can produce commodity products at the rates and yields required by industry via direct microbial conversion. However, new genomic ... -
Crispr-based multi-gene integration strategies to create saccharomyces cerevisiae strains for consolidated bioprocessing
Jacob, Odwa; van Lill, Gert Rutger; den Haan, Riaan (MDPI, 2022)Significant engineering of Saccharomyces cerevisiae is required to enable consolidated bioprocessing (CBP) of lignocellulose to ethanol. Genome modification in S. cerevisiae has been successful partly due to its efficient ... -
Identification of superior cellulase secretion phenotypes in haploids derived from natural Saccharomyces cerevisiae isolates
Davison, Steffi A.; den Haan, Riaan; van Zyl, Willem Heber (Oxford University Press, 2019)The yeast Saccharomyces cerevisiae is considered an important host for consolidated bioprocessing and the production of high titres of recombinant cellulases is required for efficient hydrolysis of lignocellulosic substrates ... -
Improved cellulase expression in diploid yeast strains enhanced consolidated bioprocessing of pretreated corn residues
den Haan, Riaan (ScienceDirect, 2019)In an effort to find a suitable genetic background for efficient cellulolytic secretion, genetically diverse strains were transformed to produce core fungal cellulases namely, β-glucosidase (BGLI), endoglucanase (EGII) and ... -
Supplementation of recombinant cellulases with LPMOs and CDHs improves consolidated bioprocessing of cellulose
Smuts, Ivy E.; den Haan, Riaan; Blakeway, Nicole J. (Elsevier, 2023)The increased demand for energy has sparked a global search for renewable energy sources that could partly replace fossil fuel resources and help mitigate climate change. Cellulosic biomass is an ideal feedstock for renewable ...