Browsing by Subject "Hyperthermophiles"
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Cowan, Donald A.; Arslanoglu, A.; Burton, Stephanie; Cameron, Rory A.; Baker, Gillian; Smith, Jacques J.; Meyer, Quinton (Portland Press, 2004)[more][less]
Abstract: With the rapid development of powerful protein evolution and enzyme-screening technologies, there is a growing belief that optimum conditions for biotransformation processes can be established without the constraints of the properties of the biocatalyst. These technologies can then be applied to find the ‘ideal biocatalyst’ for the process. In identifying the ideal biocatalyst, the processes of gene discovery and enzyme evolution play major roles. However, in order to expand the pool genes for in vitro evolution, new technologies, which circumvent the limitations of microbial culturability, must be applied. These technologies, which currently include metagenomic library screening, gene-specific amplification methods and even full metagenomic sequencing, provide access to a volume of ‘sequence space’ that is not addressed by traditional screening. URI: http://hdl.handle.net/10566/142 Files in this item: 1
CowanMetagenomicsGeneDiscovery2004.pdf (575.0Kb) -
Cowan, Donald A. (Elsevier, 2004)[more][less]
Abstract: The newly isolated hyperthermophilic archaeal strain 121 grows slowly at 121 8C and even survives short periods at 130 8C. This is another organism that grows best at temperatures well in excess of 100 8C! We should not be astonished so much by the numerical increments but by the biochemical implications of this fact, and we should be excited by the scope provided by this and similar organisms to further our understanding of the evolution and adaptation of molecular structures and systems. And what about the upper limit of life? It appears improbable that the end-point of this search is represented by strain 121. The consensus view is that the true upper limit, where the energetic burden imposed by molecular repair and resynthesis becomes unsustainable, will probably lie in the region of 140–150 8C URI: http://hdl.handle.net/10566/147 Files in this item: 1
CowanUpperTemperature2004.pdf (331.8Kb)
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