Browsing Faculty of Natural Sciences by Title
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Mugabo, Pierre; Obikezea, Kenechukwu; Njagi, Angela; Burger, Andries; Dietrich, Danielle; Green, Ivan (Open Access Science Research Publisher, 2012)[more][less]
Abstract: Crinum macowanii has been used extensively in traditional medicines for treatment of various illnesses such as oedema and ‘heart disease’. Previous studies of the crude bulb extracts on Langendorff perfused isolated rat hearts indicated a positive inotropic effect. The aim of this study was to isolate and characterize compound(s) from C. macowanii with cardiovascular effects similar to that observed with the crude extracts of the plant. The methanol extract of dried bulbs was extracted for alkaloids, and structural elucidation of the isolated alkaloid identified it as hippadine. The cardiovascular effects of hippadine was evaluated in vitro in isolated perfused rat hearts using the “double sided” working heart system. Perfusion with 0.5 μg/ml and 5.0 μg/ml hippadine in Krebs-Hanseleit buffer led to significant decreases in coronary flow, aortic output, cardiac output, systolic pressure, and heart rate, accompanied by increases in diastolic pressure. Hippadine exhibited a negative chronotropic and inotropic effect on the isolated rat heart and is responsible either partly or fully for the cardiovascular effects of C. macowanii. URI: http://hdl.handle.net/10566/401 Files in this item: 1
MugaboHippadine2012.pdf (63.78Kb) -
Gershwin, Lisa-Ann; Gibbons, Mark J. (Magnolia Press, 2009)[more][less]
Abstract: A common and conspicuous member of the medusan fauna of South Africa has been the subject of repeated taxonomic confusion. After having been mistakenly identified by earlier workers as either Carybdea alata or Tamoya haplonema, this large and colourful carybdeid is described herein as Carybdea branchi, sp. nov. It is distinguished from its congeners on numerous characters: body to about 8cm in life, densely scattered with nematocyst warts over the whole body and abaxial keels of the pedalia; a bulge or lateral thorn on the pedalial canal bend; with 2 dendritic velarial canals per octant, highly diverticulated; with a long manubrium; with greatly bushy epaulette-like phacellae, comprising 20 or more roots tightly clustered; with well developed mesenteries; and with conspicuous brownish pigmented areas over the proximal and distal regions of the pedalia and over the phacellae. A comparative table of the primary diagnostic characters of species in the genus Carybdea is given. URI: http://hdl.handle.net/10566/369 Files in this item: 1
GershwinCarybdeida2009.pdf (1.003Mb) -
Mutemwa, Muyowa; Tucker, William D.; Norman, Michael (Telkom, 2009)[more][less]
Abstract: This paper discusses how an Instant Messaging application on a computer can use Bluetooth in order to provide vibration notification on a mobile phone. The initial motivation was to aid Deaf1 office workers to know when events happened on the computer on their desks. Deaf people with access to modern technology have become accustomed to using Instant Messaging, email and video conferencing. However, most of these applications are designed for hearing users and often use audible notification. Cell phone vibration offers a way to convey similar notifications because many Deaf users have a cell phone. The use of SMS has also become widespread among Deaf users because they cannot hear or speak, even though they might be somewhat text illiterate. Vibration notification in addition to aural notification is common on most cell phones and Deaf users can use the former. This paper describes a Bluetooth notification system to notify a Deaf user with vibration on a cell phone whenever a new Instant Message is received on a given computer. A design goal was to provide an application programming interface to the notification system so that it can be used with any form of Web 2.0 desktop communication tool. URI: http://hdl.handle.net/10566/530 Files in this item: 1
MutemwaNormanTuckerBluetooth2009.pdf (343.6Kb) -
Musingarimi, Wicleffe; Tuffin, I. Marla; Cowan, Donald A. (Academy of Science of South Africa, 2010)[more][less]
Abstract: An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase), arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known arsenic resistance operons. The Bacillus sp. UWC arsRBCDA genes were shown to be arranged in an unusual manner with the arsDA genes immediately downstream of arsC. URI: http://hdl.handle.net/10566/160 Files in this item: 1
MusingarimiCharacterisation2010.PDF (566.6Kb) -
Sivakumar, Pasupathi; Maiyalagan, Thandavarayan (Trans Tech Publications, 2010)[more][less]
Abstract: Fuel cells, as devices for direct conversion of the chemical energy of a fuel into electricity by electrochemical reactions, are among the key enabling technologies for the transition to a hydrogen-based economy. Among the various types of fuel cells, polymer electrolyte membrane fuel cells (PEMFCs) are considered to be at the forefront for commercialization for portable and transportation applications because of their high energy conversion efficiency and low pollutant emission. Cost and durability of PEMFCs are the two major challenges that need to be addressed to facilitate their commercialization. The properties of the membrane electrode assembly (MEA) have a direct impact on both cost and durability of a PEMFC. An overview is presented on the key components of the PEMFC MEA. The success of the MEA and thereby PEMFC technology is believed to depend largely on two key materials: the membrane and the electro-catalyst. These two key materials are directly linked to the major challenges faced in PEMFC, namely, the performance, and cost. Concerted efforts are conducted globally for the past couple of decades to address these challenges. This chapter aims to provide the reader an overview of the major research findings to date on the key components of a PEMFC MEA. URI: http://hdl.handle.net/10566/166 Files in this item: 1
MaiyalaganPasupathi2010.pdf (1.330Mb) -
Tucker, William D. (ACM, 2004)[more][less]
Abstract: Connecting people across the Digital Divide is as much a social effort as a technological one. We are developing a community-centered approach to learn how interaction techniques can compensate for poor communication across the Digital Divide. Preliminary trials have yielded interfaces that deal with poor quality by adapting Instant Messaging techniques for multiple modalities, providing improved semi-synchronous communication. Lessons learned suggest new ways to design user interfaces specifically for the developing world. URI: http://hdl.handle.net/10566/591 Files in this item: 1
Tucker-CHI2004.pdf (81.56Kb) -
Raji, Ismaila; Mugabo, Pierre; Obikeze, Kenechukwu (BioMed Central, 2013)[more][less]
Abstract: Background: Tulbaghia violacea Harv. (Alliaceae) is used to treat various ailments, including hypertension (HTN) in South Africa. This study aims to evaluate the contributions of muscarinic receptors and changes in plasma aldosterone levels to its anti-hypertensive effect. Methods: In the acute experiments, methanol leaf extracts (MLE) of T. violacea (30–120 mg/kg), muscarine (0.16 -10 μg/kg), and atropine (0.02 - 20.48 mg/kg), and/or the vehicle (dimethylsulfoxide (DMSO) and normal saline (NS)) were respectively and randomly administered intravenously in a group of spontaneously hypertensive (SHR) weighing 300 to 350 g and aged less than 5 months. Subsequently, T. violacea (60 mg/kg) or muscarine (2.5 μg/kg) was infused into eight SHRs, 20 min after atropine (5.12 mg/kg) pre-treatment. In the chronic (21 days) experiments, the SHRs were randomly divided into three groups, and given the vehicle (0.2 ml/day of DMSO and NS), T. violacea (60 mg/kg/day) and captopril (10 mg/kg/day) respectively into the peritoneum, to investigate their effects on blood pressure (BP), heart rate (HR), and plasma aldosterone levels. Systolic BP and HR were measured using tail-cuff plethysmography during the intervention. BP and HR were measured via a pressure transducer connecting the femoral artery and the Powerlab at the end of each intervention in the acute experiment; and on day 22 in the chronic experiment. Results: In the acute experiments, T. violacea, muscarine, and atropine significantly (p < 0.05) reduced BP dose-dependently. T. violacea and muscarine produced dose-dependent decreases in HR, while the effect of atropine on HR varied. After atropine pre-treatment, dose-dependent increases in BP and HR were observed with T. violacea; while the BP and HR effects of muscarine were nullified. In the chronic experiments, the T. violaceatreated and captropril-treated groups had signicantly lower levels of aldosterone in plasma when compared to vehicle-treated group. Compared to the vehicle-treated group, significant reduction in BP was only seen in the captopril-treated group; while no difference in HR was observed among the groups. Conclusion: The results obtained in this study suggest that stimulation of the muscarinic receptors and a reduction in plasma aldosterone levels contribute to the anti-hypertesive effect of T. violacea. URI: http://hdl.handle.net/10566/600 Files in this item: 1
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Chetty, Marshini; Tucker, William D.; Blake, Edwin H. (SAICSIT, in assocation with ACM, 2004)[more][less]
Abstract: The digital divide between rural and urban areas within developing countries is vast. We investigate how to address this divide by introducing Information and Communication Technologies (ICTs) into remote rural areas. Our aim is to discover how to create locally relevant software applications with appropriate content for these areas. We use a user centred design approach and a modified software development lifecycle that is participatory, iterative and cyclical. This process is based on principles from Participatory Design and Action Research. This paper presents our initial experience of developing a telemedicine application for a rural village in the Eastern Cape Province of South Africa using this process. We present an overview of the methodology, describe the software application we have developed and cite several challenges we have faced. Finally we conclude that an inter-organisational and inter-disciplinary approach is needed to develop software for remote areas. URI: http://hdl.handle.net/10566/532 Files in this item: 1
ChettyTuckerBlakeICT2004.pdf (386.9Kb) -
Ah Tow, Lemese; Cowan, Donald A. (Springer Verlag, 2005)[more][less]
Abstract: Continental Antarctic is perceived as a largely pristine environment, although certain localized regions (e.g., parts of the Ross Dependency Dry Valleys) are relatively heavy impacted by human activities. The procedures imposed on Antarctic field parties for the handling and disposal of both solid and liquid wastes are designed to minimise eutrofication and contamination (particularly by human enteric bacteria). However, little consideration has been given to the significance, if any, of less obvious forms of microbial contamination resulting from periodic human activities in Antarctica. The predominant commensal microorganism on human skin, Staphylococcus epidermidis, could be detected by PCR, in Dry Valley mineral soils collected from heavily impacted areas, but could not be detected in Dry Valley mineral soils collected from low impact and pristine areas. Cell viability of this non-enteric human commensal is rapidly lost in Dry Valley mineral soil. However, S. epidermidis can persist for long periods in Dry Valley mineral soil as non-viable cells and/or naked DNA. URI: http://hdl.handle.net/10566/146 Files in this item: 1
AhTowDissemination2005.pdf (393.6Kb) -
Valisi, Andiswa; Maiyalagan, Thandavarayan; Khotseng, Lindiwe; Linkov, Vladimir; Pasupathi, Sivakumar (Springer, 2012)[more][less]
Abstract: This work studies the effect of heat treatment of carbon-dispersed platinum and platinum alloys on its methanol tolerance and catalytic activity as gas diffusion electrodes for oxygen reduction reaction (ORR) in acid medium. The catalyst powders were subjected to heat treatments at three different temperatures for a fixed period at controlled atmospheres. Differences in catalyst morphology were characterized using X-ray diffraction, energy dispersive X-ray analysis and transmission electron microscope techniques. The electrochemical characteristics and activity of the electro-catalysts were evaluated for ORR and methanol tolerance using cyclic voltammetry, in the form of gas diffusion electrodes. The optimum heat-treatment temperature is found to be strongly dependent on the individual catalyst. The maximum ORR activity and better methanol tolerance for the oxygen reduction reaction (ORR) was observed in Pt-Fe/C and Pt-Cu/C catalysts subjected to heat treatment at 350 °C.A trend of catalytic activity for oxygen reduction reaction (ORR) was obtained: Pt-Cu/C (350°C)>Pt-Fe/C (350°C) > Pt-Ni/C (350°C) > Pt-Co/C (250°C) > Pt/C (350°C), showing that Pt-Cu/C-type catalysts had a higher catalytic activity with reasonable methanol tolerance. URI: http://hdl.handle.net/10566/343 Files in this item: 1
ValisiHeatTreatment2012.pdf (10.92Mb) -
Mugabo, Pierre; Khan, Fatima; Burger, Andries (Open Access Science Research Publisher, 2012)[more][less]
Abstract: The use the aqueous decoction of Leonotis leonurus (L. leonurus) (Ll) R. Br. (Lamiaceae) in the treatment of hypertension (HPT) in traditional medicine is well documented. The effect of the aqueous extract of LI on the blood pressure (BP) and heart rate (HR) has been investigated in normotensive rats. The aim of this study was to investigate the effect of Ll aqueous extract on the in isolated perfused rat heart (IPRH). Hearts were excised from male Wistar albino rats weighing 250-350g, aged less than 6 months. They were perfused at constant flow using the modified Langendorff perfused model of the heart. Effects of adrenaline on the left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (LVDP), heart rate (HR), cardiac work (CW) and coronary perfusion pressure (CPP) were compared to that of Ll. Adrenaline (1µM) significantly (p<0.05) increased the LVSP by 40.6%, the LVDP by 43.9%, the HR by 22.5% and the CW by 89.4%. Ll (1.0 mg/ml and 2.0 mg/ml respectively and significantly (p<0.01) increased the LVSP by 25.36 and 14.91, the LVDP by 29.40 and 14.88. Ll (1.0 mg/ml and 2.0 mg/ml) significantly produced a negative chronotropic effect. Both adrenaline and Ll aqueous extract did not have any significant effect on the LVEDP. Adrenaline resulted in positive inotropic and chronotropic effects. At low concentrations Ll produced a positive inotropic and a negative chronotropic effect. At the concentration of 2.0mg/ml Ll decreased all parameters to zero. At higher concentrations higher than 2.0mg/ml, Ll seemed to have toxic effects on the heart. URI: http://hdl.handle.net/10566/400 Files in this item: 1
MugaboLeonotisLeonurus2012.pdf (362.4Kb) -
Wilkinson, Dianne E.; Jaenicke, Thomas; Cowan, Donald A. (Kluwer Academic Publishers, 2002)[more][less]
Abstract: An efficient and simple method for constructing an environmental library using mechanically sheared DNA obtained directly from geothermal sediments is presented. The method is based on blunt-end modification of DNA fragments followed by 3' -adenylation using Vent DNA polymerase and Taq DNA polymerase, respectively. The prepared DNA fragments are then ligated into a TA cloning vector and used in the transformation of Escherichia coli. This method has been successfully applied to the cloning of ORFs derived from uncultivated prokaryotes present in geothermal sediment. URI: http://hdl.handle.net/10566/161 Files in this item: 1
WilkinsonEfficientMolecular2002.pdf (754.4Kb) -
Cowan, Donald A.; Ah Tow, Lemese (Annual Reviews, 2004)[more][less]
Abstract: The Antarctic continent harbors a range of specialized and sometimes highly localized microbial biotopes. These include biotopes associated with desiccated mineral soils, rich ornithogenic soils, glacial and sea ice, ice-covered lakes, translucent rocks, and geothermally heated soils. All are characterized by the imposition of one or more environmental extremes (including low temperature, wide temperature fluctuations, desiccation, hypersalinity, high periodic radiation fluxes, and low nutrient status). As our understanding of the true microbial diversity in these biotopes expands from the application of molecular phylogenetic methods, we come closer to the point where we can make an accurate assessment of the impacts of environmental change, human intervention, and other natural and unnatural impositions. At present, it is possible to make reasonable predictions about the physical effects of local climate change, but only general predictions on possible changes in microbial community structure. The consequences of some direct human impacts, such as physical disruption of microbial soil communities, are obvious if not yet quantitated. Others, such as the dissemination of nonindigenous microorganisms into indigenous microbial communities, are not yet understood. URI: http://hdl.handle.net/10566/145 Files in this item: 1
Endangered Antarctic Environments.docx (12.30Kb) -
Adesina, Ademola O.; Agbele, Kehinde K.; Februarie, Ronald; Abidoye, Ademola P.; Nyongesa, Henry O. (Academy of Science of South Africa, 2011)[more][less]
Abstract: The sensitivity of health-care information and its accessibility via the Internet and mobile technology systems is a cause for concern in these modern times. The privacy, integrity and confidentiality of a patient’s data are key factors to be considered in the transmission of medical information for use by authorised health-care personnel. Mobile communication has enabled medical consultancy, treatment, drug administration and the provision of laboratory results to take place outside the hospital. With the implementation of electronic patient records and the Internet and Intranets, medical information sharing amongst relevant health-care providers was made possible. But the vital issue in this method of information sharing is security: the patient’s privacy, as well as the confidentiality and integrity of the health-care information system, should not be compromised. We examine various ways of ensuring the security and privacy of a patient’s electronic medical information in order to ensure the integrity and confidentiality of the information. URI: http://hdl.handle.net/10566/279 Files in this item: 1
AdesinaSecurity2011.pdf (453.4Kb) -
Hersh, Marion A.; Tucker, William D. (Elsevier, 2005)[more][less]
Abstract: There are a number of pressures on researchers in academia and industry to behave unethically or compromise their ethical standards, for instance in order to obtain funding or publish frequently. In this paper a case study of Deaf telephony is used to discuss the pressures to unethical behaviour in terms of withholding information or misleading participants that can result from mono-disciplinary orthodoxies. The Deaf telephony system attempts to automate multiple aspects of relayed communication between Deaf and hearing users. The study is analysed in terms of consequentialist and deontological ethics, as well as multi-loop action learning. Discussion of a number of examples of bad practice is used to indicate both the compatibility of ethical behaviour and good scientific method and that ethical behaviour is a pre-requisite for obtaining meaningful results. URI: http://hdl.handle.net/10566/466 Files in this item: 1
HershTuckerUnethicalBehaviour2005.pdf (86.66Kb) -
Gibbons, Mark J.; Stuart, V (NISC and Taylor & Francis, 1994)[more][less]
Abstract: The feeding biology and the vertical migration of Sagitta friderici were examined over 24 h at two stations in the southern Benguela during spring (October) 1987. Together with studies conducted during summer (February 1991) and winter (May 1984), they serve to allow valuable generalizations of the biology and ecology of this abundant chaetognath. Populations migrate vertically and feed nocturnally, although the timing and the extent of migration vary between studies. S.friderici exhibits ontogenetic layering and the cross-shelf distribution of maturity stages differs, suggesting that it is able to take advantage of cross-shelf water movement in order to maintain populations in the nearshore waters of the West Coast. S.friderici prey almost exclusively on copepodg (cannibalism is rare), and there is a positive relationship between the lengths of predator and prey that is influenced by the size structure of the prey environment. This casts doubt on the validity of a chaetognath species-specific relationship between predator and prey size. S. friderici selects its prey on the basis of size, and not species. Daily ration is related linearly to prey density, so reflecting the low density of prey and providing support for theoretical predictions regarding ingestion rates under oceanic conditions. The impact of S. friderici predation on the copepod assemblage is generally less than 3% of the standing stock, although it could be much higher under conditions of low copepod biomass and poor secondary production. URI: http://hdl.handle.net/10566/306 Files in this item: 1
GibbonsFeeding1994.pdf (866.4Kb) -
Chitedze, Zimani; Tucker, William D. (Telkom, 2012)[more][less]
Abstract: This paper shows that mobility management protocols for infrastructure Internet may be used in a wireless mesh network environment. Mesh topology tends to be an unplanned graph and routes change dynamically and in this research Mobile IPv6 and Fast Handover for Hierarchical Mobile IPv6 are successfully implemented in a wireless mesh network environment. Horizontal handover simulation with ns2 involved Mobile IPv6 and Fast Handover for Hierarchical Mobile IPv6 applied to wireless mesh networks. Mobile IPv6 was used as a baseline to compare the performance of the two protocols. The results show that in mesh networks, Fast Handover for Hierarchical Mobile IPv6‘s performance is superior to Mobile IPv6. Fast Handover for Hierarchical Mobile IPv6 generates more throughput and less delay than Mobile IPv6. Furthermore, Fast Handover for Hierarchical Mobile IPv6 drops fewer data packets than Mobile IPv6. Even though MIPv6 and its extensions are for infrastructure networks, they can be used effectively in mesh networks. URI: http://hdl.handle.net/10566/424 Files in this item: 1
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Benjamin, Anita; Tucker, William D. (Telkom, 2003)[more][less]
Abstract: We have built a hybrid voice/text web–board that utilizes floor control for handling resources such as microphone and interface events. The aim of this research is to determine the most appropriate floor control mechanism for a hybrid communication tool that allows conventional text and audio users to communicate with one another using a common interface. Research on floor control mechanisms provides many implementations for arbitration that are application-specific. Myers proposes a categorical explanation of the most widely used mechanisms for providing floor control and discusses these different dimensions with respect to a project called Pebbles. We have chosen to model our arbitration policies along these categories. The application will be implemented as a Java applet and gives users a view of both the text and audio aspects of the exchange. To facilitate the multi-modality of the system we will provide media conversion, using the Nuance system’s Java API. Our hypothesis is that well–known multi-tasking arbitration protocols such as FCFS, Round Robin, Token – based, and many more can be adapted to create a conferencing floor control policy for the interface of a hybrid communication environment so as to facilitate free–flowing multi-modal conversation. Measurements to determine which floor control implementation provides the best arbitration in terms of user interaction with the system will be calculated using RPT, ORT and usage tests based on the Kirkpatrick Model. We hope to show that users benefit more from an implicit arbitration mechanism than they do from an explicit one. URI: http://hdl.handle.net/10566/538 Files in this item: 1
BenjaminTuckerHybridVoice2003.pdf (123.3Kb) -
Tucker, William D.; Keats, Derek (Telkom, 1998)[more][less]
Abstract: Grafting an ATM network onto an existing Ethernet network is a non-trivial exercise in network configuration. The process of mating ATM technology to an Ethernet network without tampering the underlying backbone and network configuration gives rise to a variety of networking issues including addressing, compatibility, security, efficiency, administrative effort, and scalability. Examples of actual situations serve to illustrate these issues. We have designed several scenarios to tackle the topological problems of an ATM graft. A dual IP approach works but suffers from a lack of scalability. We expect an edge routing solution, with subnetting, will be the most elegant and scalable. URI: http://hdl.handle.net/10566/448 Files in this item: 1
TuckerKeatsATM1998.pdf (612.2Kb) -
Julius, Elroy P.; Tucker, William D. (Telkom, 2005)[more][less]
Abstract: This paper presents an empirical solution for guaranteeing the delivery of synchronous and asynchronous messages within a semi-synchronous IPbased communication domain. The communication infrastructure that is needed between Deaf and hearing communities forms the application domain within which the research is situated. SoftBridging is a framework for multi-modal bridging as well as multi-user, multi-modal conversation sessions. An implementation of this concept called SoftBridge for Instant Messaging Bridging Application (SIMBA), is a communication platform that allows a hearing and Deaf person to communicate inside a single uniform space. The system makes use of various web services to do the actual data conversions such as voice to text and text to voice. Publish-subscribe systems are an emerging paradigm for building a range of distributed applications. The architecture of publishsubscribe systems make use of Message Oriented Middleware (MOM) to guarantee reliable delivery of messages within a communication domain. We have incorporated a publish-subscribe system within the overall architecture of SIMBA. We have thus modified the existing architecture of SIMBA to reliably transport semi-synchronous data over a synchronous established session. We have chosen to use the Session Initiation Protocol (SIP) for the establishment of a synchronous session between various users and SIMBA. The system is currently being used as a basis for developing a Deaf telephony application that guarantees the delivery of messages no matter the synchrony. URI: http://hdl.handle.net/10566/509 Files in this item: 1
JuliusTuckerSoftBridging2005.pdf (589.9Kb)