Browsing Faculty of Natural Sciences by Subject "Biotechnology"
Now showing items 21-40 of 59
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Establishing minion sequencing and genome assembly procedures for the analysis of the rooibos (aspalathus linearis) genome
(MDPI, 2022)While plant genome analysis is gaining speed worldwide, few plant genomes have been sequenced and analyzed on the African continent. Yet, this information holds the potential to transform diverse industries as it unlocks ... -
Extremophiles in biofuel synthesis
(Taylor and Francis Group, 2010)The current global energy situation has demonstrated an urgent need for the development of alternative fuel sourcesto the continually diminishing fossil fuel reserves. Much research to address this issue focuses on the ... -
Flavonoid and phenolic acid profiles of dehulled and whole vigna subterranea (l.) Verdc seeds commonly consumed in South Africa
(MDPI, 2022)Bambara groundnut (BGN) is an underexploited crop with a rich nutrient content and is used in traditional medicine, but limited information is available on the quantitative characterization of its flavonoids and phenolic ... -
Functional prediction of candidate micrornas for CRC management using in silico approach
(MDPI, 2019)Approximately 30–50% of malignant growths can be prevented by avoiding risk factors and implementing evidence-based strategies. Colorectal cancer (CRC) accounted for the second most common cancer and the third most common ... -
Genetic characterization of the central variable region in African swine fever virus isolates in the Russian federation from 2013 to 2017
(MDPI, 2022)African swine fever virus (ASFV), classified as genotype II, was introduced into Georgia in 2007, and from there, it spread quickly and extensively across the Caucasus to Russia, Europe and Asia. The molecular epidemiology ... -
Genetic polymorphisms and haplotypes of the organic cation transporter 1 gene (SLC22A1) in the Xhosa population of South Africa
(Brazilian Society of Genetics, 2014)Human organic cation transporter 1 is primarily expressed in hepatocytes and mediates the electrogenic transport of various endogenous and exogenous compounds, including clinically important drugs. Genetic polymorphisms ... -
Genetic variation in responses to salt stress in Tunisian populations of Medicago ciliaris
(MDPI, 2022)Soil salinity is one of the most serious environmental factors affecting crop productivity around the world. We used a morpho-physiological approach to investigate the salt responses of four Tunisian natural populations ... -
GlobalFiler® express DNA amplification kit in South Africa: Extracting the past from the present
(Elsevier, 2016)In this study, the GlobalFiler1 Express amplification kit was evaluated for forensic use in 541 South African individuals belonging to the Afrikaaner, amaXhosa,1 amaZulu,1 Asian Indian and Coloured population groups. Allelic ... -
Green synthesis of iron oxide (hematite) nanoparticles and their influence on sorghum bicolor growth under drought stress
(MDPI, 2023)Drought is a major abiotic stress that confronts plant growth and productivity, thus compromising food security. Plants use physiological and biochemical mechanisms to cope with drought stress, but at the expense of ... -
Green-synthesized zinc oxide nanoparticles mitigate salt stress in sorghum bicolor
(MDPI, 2022)Salinity is an abiotic stress that is responsible for more than 50% of crop losses worldwide. Current strategies to overcome salinity in agriculture are limited to the use of genetically modified crops and chemicals ... -
A guide to molecular characterization of genotype ii African swine fever virus: Essential and alternative genome markers
(MDPI, 2023)African swine fever is a contagious viral disease that has been spreading through Europe and Asia since its initial report from Georgia in 2007. Due to the large genome size of the causative agent, the African swine fever ... -
Hypoglycaemic activity of biosynthesized copper oxide nanoparticles in alloxan-induced diabetic Wister rats
(Wiley, 2023)Diabetes mellitus (DM) is a metabolic disorder that affects the body's ability to produce or use insulin. This study evaluated the hypoglycaemic activity of biosynthesized copper oxide nanoparticles (CuO-NPs) ... -
Identification and characterization of a heat-inducible Hsp70 gene from Sorghum bicolor which confers tolerance to thermal stress
(Springer, 2015)This study describes the first detailed molecular characterization of the heat shock protein 70 (Hsp70) gene from Sorghum bicolor, MN1618 designated as SbHsp70-1. The full-length cDNA of SbHsp70-1 consists of 2524 bp ... -
Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from Antarctic desert soil
(American Society for Microbiology, 2009)The cold deserts of the McMurdo Dry Valleys, South Victoria Land, Eastern Antarctica, are widely acknowledged as having the harshest soil environments on Earth (6, 8, 26). Despite the apparent hostility of the environment, ... -
Identification of a novel protein with guanylyl cyclase activity in Arabidopsis thaliana
(Elsevier, 2003)Guanylyl cyclases (GCs) catalyze the formation of the second messenger guanosine 3 ,5 -cyclic monophos- phate (cGMP) from guanosine 5 -triphosphate (GTP). While many cGMP-mediated processes in plants have been reported, ... -
Identification of superior cellulase secretion phenotypes in haploids derived from natural Saccharomyces cerevisiae isolates
(Oxford University Press, 2019)The yeast Saccharomyces cerevisiae is considered an important host for consolidated bioprocessing and the production of high titres of recombinant cellulases is required for efficient hydrolysis of lignocellulosic substrates ... -
Impact of metagenomic DNA extraction procedures on the identifiable endophytic bacterial diversity in Sorghum bicolor (L. Moench)
(Elsevier, 2015)Culture-independent studies rely on the quantity and quality of the extracted environmental metagenomic DNA (mDNA). To fully access the plant tissue microbiome, the extracted plant mDNA should allow optimal PCR applications ... -
In silico prediction of new antimicrobial peptides and proteins as druggable targets towards alternative anti-schistosomal therapy
(Elsevier, 2021)Schistosomiasis is a debilitating disease caused by a parasitic flatworm found in fresh- water. With the exponential increase in prevalence, Praziquantel (PZQ) remains the only effective treatment drug, however, resistance ... -
Increasing extracellular cellulase activity of the recombinant Saccharomyces cerevisiae by engineering cell wall-related proteins for improved consolidated processing of carbon neutral lignocellulosic biomass
(Elsevier, 2022)Sustainable bioproduction using carbon neutral feedstocks, especially lignocellulosic biomass, has attracted increasing attention due to concern over climate change and carbon reduction. Consolidated bioprocessing (CBP) of ... -
Isolation and characterization of lytic proteus virus 309
(MDPI, 2022)Proteus mirabilis is frequently associated with complicated urinary tract infections (UTIs) and is the main cause of catheter-associated urinary tract infections (CAUTIs). Treatment of such infections is complicated and ...