Browsing Institute for Microbial Biotechnology & Metagenomics by Author "Burton, Stephanie"
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Cowan, Donald A.; Arslanoglu, A.; Burton, Stephanie; Cameron, Rory A.; Baker, Gillian; Smith, Jacques J.; Meyer, Quinton (Portland Press, 2004)[more][less]
Abstract: With the rapid development of powerful protein evolution and enzyme-screening technologies, there is a growing belief that optimum conditions for biotransformation processes can be established without the constraints of the properties of the biocatalyst. These technologies can then be applied to find the ‘ideal biocatalyst’ for the process. In identifying the ideal biocatalyst, the processes of gene discovery and enzyme evolution play major roles. However, in order to expand the pool genes for in vitro evolution, new technologies, which circumvent the limitations of microbial culturability, must be applied. These technologies, which currently include metagenomic library screening, gene-specific amplification methods and even full metagenomic sequencing, provide access to a volume of ‘sequence space’ that is not addressed by traditional screening. URI: http://hdl.handle.net/10566/142 Files in this item: 1
CowanMetagenomicsGeneDiscovery2004.pdf (575.0Kb) -
Burton, Stephanie; Cowan, Donald A.; Woodley, John M. (Nature Publishing Group, 2002)[more][less]
Abstract: While the use of enzymes as biocatalysts to assist in the industrial manufacture of fine chemicals and pharmaceuticals has enormous potential, application is frequently limited by evolution-led catalyst traits. The advent of designer biocatalysts, produced by informed selection and mutation through recombinant DNA technology, enables production of process-compatible enzymes. However, to fully realize the potential of designer enzymes in industrial applications, it will be necessary to tailor catalyst properties so that they are optimal not only for a given reaction but also in the context of the industrial process in which the enzyme is applied. URI: http://hdl.handle.net/10566/150 Files in this item: 1
BurtonCowanWoodley2002.pdf (768Kb)
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