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Cloning, expression and characterization of thermostable YdaP from Bacillus licheniformis 9A
(Acta Biochimica Polonica, 2017)
The Bacillus licheniformis ydaP gene encodes for a pyruvate
oxidase that catalyses the oxidative decarboxylation
of pyruvate to acetate and CO2. The YdaP form of
this enzyme was purified about 48.6-folds to homogeneity
in ...
Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus
(BioMed Central, 2014)
BACKGROUND: Bacterial pyruvate decarboxylases (PDC) are rare. Their role in ethanol production and in bacterially
mediated ethanologenic processes has, however, ensured a continued and growing interest. PDCs from ...
Virome assembly and annotation: A surprise in the Namib Desert
(Frontiers Research Foundation, 2017)
Sequencing, assembly, and annotation of environmental virome samples is challenging. Methodological biases and differences in species abundance result in fragmentary read coverage; sequence reconstruction is further ...
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils
(American Society for Microbiology, 2014)
The metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities,
cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded ...
Selection of Clostridium spp. in biological sand filters neutralizing synthetic acid mine drainage
(Wiley, 2013)
In this study, three biological sand filter (BSF) were contaminated with a synthetic iron- [1500 mg L-1 Fe(II), 500 mg L-1 Fe(III)] and sulphate-rich (6000 mg L-1 SO2/4-) acid mine drainage (AMD) (pH = 2), for 24 days, to ...
Draft Genome Sequence of Gordonia lacunae BS2T
(American Society for Microbiology, 2017)
We report here the draft genome sequence of the soil bacterium Gordonia
lacunae BS2T ( DSM 45085T JCM 14873T NRRL B-24551T), isolated from
an estuary in Plettenberg Bay, South Africa. Analysis of the draft genome ...
Characterisation of campylobacter concisus strains from South Africa using amplified fragment length polymorphism (AFLP) profiling and a genomospecies-specific polymerase chain reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical data
(Academic Journals, 2013)
Amplified Fragment Length Polymorphism (AFLP) profiling was used to evaluate the distribution of
phenotypically indistinguishable, but genetically distinct, among Campylobacter concisus strains from
South Africa. A Polymerase ...
A designed experiments approach to optimizing MALDI-TOF MS spectrum processing parameters enhances detection of antibiotic resistance in Campylobacter jejuni
(Frontiers Media, 2016)
MALDI-TOF MS has been utilized as a reliable and rapid tool for microbial fingerprinting at the genus and species levels. Recently, there has been keen interest in using MALDI-TOF MS beyond the genus and species levels to ...
Molecular characterization of the 16S rRNA Gene of helicobacter fennelliae isolated from stools and blood cultures from paediatric patients in South Africa
(Hindawi, 2011)
Forty strains of H. fennelliae collected from paediatric blood and stool samples over an 18 year period at a children's hospital in
Cape Town, South Africa, were amplified by PCR of the 16S rRNA. Two distinct genotypes ...
Multilocus sequence typing methods for the emerging Campylobacter species C. hyointestinalis, C. lanienae, C. sputorum, C. concisus, and C. curvus
(Frontiers Media, 2012)
Multilocus sequence typing (MLST) systems have been reported previously for multiple food - and food animal-associated Campylobacter species (e.g., C. jejuni, C. coli, C. lari, and C. fetus) to both differentiate strains ...