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Now showing items 181-190 of 193
A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye
(Future Science Group, 2008)
We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated ...
Development of infectious transcripts and genome manipulation of black queen-cell virus of honey bees
(Microbiology Society, 2002)
The South African isolate of Black queen-cell virus (BQCV), a honey bee virus, was previously found
to have an 8550 nucleotide genome excluding the poly(A) tail. Its genome contained two ORFs, a
5«-proximal ORF encoding ...
FRAGS: Estimation of coding sequence substitution rates from fragmentary data
(BMC, 2004)
Rates of substitution in protein-coding sequences can provide important insights into evolutionary processes that are of biomedical and theoretical interest. Increased availability of coding sequence data has enabled ...
The contribution of exon-skipping events on chromosome 22 to protein coding diversity
(Cold Spring Harbor Laboratory Press, 2001)
Completion of the human genome sequence provides evidence for a gene count with lower bound 30,000–40,000. Significant protein complexity may derive in part from multiple transcript isoforms. Recent EST based studies have ...
Ozone and nitric oxide induce cGMP-dependent and independent transcription of defence genes in tobacco
(Wiley, 2009)
Here, we analyse the temporal signatures of ozone (O3)-induced hydrogen peroxide
(H2O2) and nitric oxide (NO) and the role of the second messenger guanosine
3′,5′-cyclic monophosphate (cGMP) in transcriptional changes ...
Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from Antarctic desert soil
(American Society for Microbiology, 2009)
The cold deserts of the McMurdo Dry Valleys, South Victoria Land, Eastern Antarctica, are widely acknowledged as
having the harshest soil environments on Earth (6, 8, 26).
Despite the apparent hostility of the environment, ...
2-phenoxyethanol as anaesthetic in removing and relocating 102 species of fishes representing 30 families from sea world to uShaka Marine World, South Africa
(AOSIS, 2008)
The South African Association for Marine Biological
Research, incorporating Sea World, Oceanographic
Research Institute and the Dolphinarium relocated
from their old premises of 46 years on Durban’s
beach front in March ...
Promoting physics and development in Africa
(American Institute of Physics, 2004)
To excel in physics research in Africa is to conquer Mount Everest without the aid of additional oxygen. In a continent that lacks the infrastructure of research laboratories, technical support, and so forth, relatively ...
Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form
(Wiley, 2007)
Nitrilases convert nitriles to the corresponding carboxylic acids and ammonia.
The nitrilase from Rhodococcus rhodochrous J1 is known to be inactive
as a dimer but to become active on oligomerization. The recombinant
enzyme ...
Electrocatalytic sensor devices: (I) cyclopentadienylnickel(II) thiolato Schiff base monolayer self-assembled on gold
(Elsevier, 2004)
The fabrication of a self-assembled monolayer (SAM) of a cyclopentadienylnickel(II) thiolato Schiff base compound, [Ni(SC6H4NC(H)C6H4
OCH2CH2SMe)( 5-C5H5)]2 on a gold electrode is described. Effective electronic communication ...