Calibration of redox potential in sperm wash media and evaluation of oxidation–reduction potential values in various assisted reproductive technology culture media using MiOXSYS system
Panner Selvam, M. K.
MetadataShow full item record
Oxidation–reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to support the development of fertilized oocytes under in vitro conditions. The composition and conditions of these media are vital for optimal functioning of the gametes. Currently, there are no data on the status of redox potential of sperm processing and assisted reproduction media. The purpose of this study was to compare the oxidation–reduction potential values of the different media and to calibrate the oxidation–reduction potential values of the sperm wash medium using oxidative stress inducer cumene hydroperoxide and antioxidant ascorbic acid. Redox potential was measured in 10 different media ranging from sperm wash media, freezing media and assisted reproductive technology one-step medium to sequential media. Oxidation–reduction potential values of the sequential culture medium and one-step culture medium were lower and significantly different (p < 0.05) from the sperm wash media. Calibration of the sperm wash media using the oxidant cumene hydroperoxide and antioxidant ascorbic acid demonstrated that oxidation–reduction potential and the concentration of oxidant or antioxidant are logarithmically dependent. This study highlights the importance of calibrating the oxidation–reduction potential levels of the sperm wash media in order to utilize it as a reference value to identify the physiological range of oxidation oxidation–reduction potential that does not have any adverse effect on normal physiological sperm function.
Showing items related by title, author, creator and subject.
Scharnick-Udemans, Lee-Shae S. (Association for the Study of Religion in Southern Africa, 2018)In keeping with trends in the academy and the rapidly increasing presence, power, and persuasion of digital and electronic media on the African continent and in the global economy, the study of religion and the media in ...
Knoetze, Samantha; Jantjies, Mmaki (university of western cape, 2019)Knowledge transfer of both tacit and explicit knowledge within an organization forms a crucial component of organizational continuity. In the age of technology, the manner in which knowledge is transferred from one employee ...
Makwela, Mologadi (José Frantz, 2020)Research impact is primarily measured by publication in high-ranking journals, the majority of which are in English. This narrow measurement focusing on scientific journals, together with exclusionary language policies, impedes ...