Browsing Research Articles (Biotechnology) by Title
Now showing items 120-139 of 215
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In silico identification of micrornas as candidate Colorectal cancer biomarkers
(Sage Journals, 2019)The involvement of microRNA in cancers plays a significant role in their pathogenesis. Specific expressions of these non-coding RNAs also serve as biomarkers for early colorectal cancer diagnosis, but their laboratory/molecular ... -
In silico prediction of new antimicrobial peptides and proteins as druggable targets towards alternative anti-schistosomal therapy
(Elsevier, 2021)Schistosomiasis is a debilitating disease caused by a parasitic flatworm found in fresh- water. With the exponential increase in prevalence, Praziquantel (PZQ) remains the only effective treatment drug, however, resistance ... -
In vitro Antidiabetic and Antioxidant effects of different extracts of Catharanthus roseus and its indole alkaloid, vindoline
(MPDI, 2020)The Catharanthus roseus plant has been used traditionally to treat diabetes mellitus. Scientific evidence supporting the antidiabetic effects of this plant’s active ingredient-vindoline has not been fully evaluated. In ... -
In vitro evaluation of the anti-diabetic potential of aqueous acetone Helichrysum petiolare extract (AAHPE) with molecular docking relevance in diabetes mellitus
(MDPI, 2021-12)Diabetes mellitus (DM) is a chronic metabolic condition that can lead to significant complications and a high fatality rate worldwide. Efforts are ramping up to find and develop novel α-glucosidase and α-amylase inhibitors ... -
An in vitro study on the combination effect of metformin and n-acetyl cysteine against hyperglycaemia-induced cardiac damage
(MDPI - Publisher of Open Access Journals, 2019)Chronic hyperglycaemia is a major risk factor for diabetes-induced cardiovascular dysfunction. In a hyperglycaemic state, excess production of reactive oxygen species (ROS), coupled with decreased levels of glutathione, ... -
In-vitro cytotoxicity, anti-bacterial and anti-inflammatory activities of ecklonia radiataand jania verrucosafrom Eastern Cape, South Africa
(University of Benin, 2022)Seaweeds are marine floras constituting in part to over 90% of the oceanic biomass. Despite the enrichments of seaweeds, their potentials are largely unexplored. Hence, this study evaluated the phytochemical screening, ... -
Increasing extracellular cellulase activity of the recombinant Saccharomyces cerevisiae by engineering cell wall-related proteins for improved consolidated processing of carbon neutral lignocellulosic biomass
(Elsevier, 2022)Sustainable bioproduction using carbon neutral feedstocks, especially lignocellulosic biomass, has attracted increasing attention due to concern over climate change and carbon reduction. Consolidated bioprocessing (CBP) of ... -
Indigenous African cereal crops can contribute to mitigation of the impact of climate change on food security
(Arid land institute, 2021)Zea mays L. (maize) is one of the top three cultivated cereals globally, along with wheat and rice. The United States, China, and Brazil are among the largest producers of maize, producing approximately 79% of the world’s ... -
Inhibition of bacteria associated with wound infection by biocompatible green synthesized gold nanoparticles from South African plant extracts
(MDPI, 2017)Unlike conventional physical and chemical methods, the biogenic synthesis of gold nanoparticles (GNPs) is considered a green and non-toxic approach to produce biocompatible GNPs that can be utilized in various biomedical ... -
Inhibition of NOS- like activity in maize alters the expression of genes involved in H2O2 scavenging and glycine betaine biosynthesis
(Nature Publishing Group, 2018)Nitric oxide synthase-like activity contributes to the production of nitric oxide in plants, which controls plant responses to stress. This study investigates if changes in ascorbate peroxidase enzymatic activity and ... -
Inhibitory potential of repurposed drugs against the SARS-CoV-2 main protease: A computational-aided approach
(Taylor and Francis, 2020)The exponential increase in cases and mortality of coronavirus disease (COVID-19) has called for a need to develop drugs to treat this infection. Using in silico and molecular docking approaches, this study investigated ... -
INPPO actions and recognition as a driving force for progress in plant proteomics: Change of guard, INPPO update, and upcoming activities
(Wiley, 2013)The International Plant Proteomics Organization (INPPO) is a non-profit organization whose members are scientists involved or interested in plant proteomics. Since the publication of the first INPPO highlights in 2012, ... -
Investigation of bioactive compounds in Crassocephalum rubens leaf and in vitro anticancer activity of its biosynthesized gold nanoparticles
(Elsevier, 2020)The development of cancer therapies has become difficult due to high metastasis, and lack of tissue selectivity, which in most cases affects normal cells. Demand for anticancer therapy is therefore increasing on daily ... -
Investigation of the anti-cancer and apoptotic properties of aqueous extract from fermented African locust bean seeds
(Rynnye Lyan Resources, 2021)Some fermented foods are reported to possess anti-cancer properties. Fermented African locust bean seeds is a condiment prepared from fermentation of Parkia biglobosa. It has been reportedly functional for various medicinal ... -
Isolation and characterization of lytic proteus virus 309
(MDPI, 2022)Proteus mirabilis is frequently associated with complicated urinary tract infections (UTIs) and is the main cause of catheter-associated urinary tract infections (CAUTIs). Treatment of such infections is complicated and ... -
Isolation and characterization of strain exiguobacterium sp. Krl4, a producer of bioactive secondary metabolites from a Tibetan glacier
(MPDI, 2021)Extremophilic microorganisms represent a unique source of novel natural products. Among them, cold adapted bacteria and particularly alpine microorganisms are still underexplored. Here, we describe the isolation and ... -
A label-free gold nanoparticles-based optical aptasensor for the detection of retinol binding protein 4
(MDPI, 2022)Retinol-binding protein 4 (RBP4) has been implicated in insulin resistance in rodents and humans with obesity and T2DM, making it a potential biomarker for the early diagnosis of T2DM. However, diagnostic tools for ... -
Large Scale Screening of Southern African Plant Extracts for the Green Synthesis of Gold Nanoparticles Using Microtitre-Plate Method
(Molecules, 2016-11)The preparation of gold nanoparticles (AuNPs) involves a variety of chemical and physical methods. These methods use toxic and environmentally harmful chemicals. Consequently, the synthesis of AuNPs using green chemistry ... -
A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye
(Future Science Group, 2008)We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated ... -
The macrophage response to mycobacterium tuberculosis and opportunities for autophagy inducing nanomedicines for Tuberculosis therapy
(Frontiers Media S.A., 2021)The major causative agent of tuberculosis (TB), i.e., Mycobacterium tuberculosis (Mtb), has developed mechanisms to evade host defense responses and persist within host cells for prolonged periods of time. Mtb is also ...