Browsing Research Articles (Biotechnology) by Issue Date
Now showing items 1-20 of 233
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Synthesis of biogenic gold nanoparticles from terminalia mantaly extracts and the evaluation of their in vitro cytotoxic effects in cancer cells
(MDPI, 202-09-12)Scientists have demonstrated the potential of plant materials as 'green' reducing and stabilizing agents for the synthesis of gold nanoparticles (AuNPs) and opened new ecofriendly horizons to develop effective and less ... -
Development of infectious transcripts and genome manipulation of black queen-cell virus of honey bees
(Microbiology Society, 2002)The South African isolate of Black queen-cell virus (BQCV), a honey bee virus, was previously found to have an 8550 nucleotide genome excluding the poly(A) tail. Its genome contained two ORFs, a 5«-proximal ORF encoding ... -
Identification of a novel protein with guanylyl cyclase activity in Arabidopsis thaliana
(Elsevier, 2003)Guanylyl cyclases (GCs) catalyze the formation of the second messenger guanosine 3 ,5 -cyclic monophos- phate (cGMP) from guanosine 5 -triphosphate (GTP). While many cGMP-mediated processes in plants have been reported, ... -
Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form
(Wiley, 2007)Nitrilases convert nitriles to the corresponding carboxylic acids and ammonia. The nitrilase from Rhodococcus rhodochrous J1 is known to be inactive as a dimer but to become active on oligomerization. The recombinant enzyme ... -
Alteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of methanolic extract of Commelina benghalensis (Commelinaceae) in Jurkat T cells
(Academic Journals, 2008)Stem extracts of Commelina benghalensis (Linn.), although not extensively documented, are frequently used in traditional medicine for the treatment of ailments such as skin malformations and outgrowths. Accordingly, the ... -
A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye
(Future Science Group, 2008)We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated ... -
Ozone and nitric oxide induce cGMP-dependent and independent transcription of defence genes in tobacco
(Wiley, 2009)Here, we analyse the temporal signatures of ozone (O3)-induced hydrogen peroxide (H2O2) and nitric oxide (NO) and the role of the second messenger guanosine 3′,5′-cyclic monophosphate (cGMP) in transcriptional changes ... -
Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from Antarctic desert soil
(American Society for Microbiology, 2009)The cold deserts of the McMurdo Dry Valleys, South Victoria Land, Eastern Antarctica, are widely acknowledged as having the harshest soil environments on Earth (6, 8, 26). Despite the apparent hostility of the environment, ... -
Extremophiles in biofuel synthesis
(Taylor and Francis Group, 2010)The current global energy situation has demonstrated an urgent need for the development of alternative fuel sourcesto the continually diminishing fossil fuel reserves. Much research to address this issue focuses on the ... -
The arabidopsis wall associated kinase-like 10 gene encodes a functional guanylyl cyclase and is coexpressed with pathogen defense related genes
(Public Library of Science, 2010)Second messengers have a key role in linking environmental stimuli to physiological responses. One such messenger, guanosine 3′,5′-cyclic monophosphate (cGMP), has long been known to be an essential signaling molecule in ... -
Mapping and characterisation of the sorghum cell suspension culture secretome
(Academic Journals, 2011)Here we reported the first secretomic study of sorghum (Sorghum bicolor), a naturally drought tolerant cereal crop. In this study, we used a gel-based proteomic approach in combination with mass spectrometry to separate ... -
Proteomic analysis of grape berry cell cultures reveals that developmentally regulated ripening related processes can be studied using cultured cells
(Public Library of Science, 2011)This work describes a proteomics profiling method, optimized and applied to berry cell suspensions to evaluate organ-specific cultures as a platform to study grape berry ripening. Variations in berry ripening within a ... -
Unique aliphatic amidase from a psychrotrophic and haloalkaliphilic nesterenkonia isolate
(American Society for Microbiology, 2011)Nesterenkonia strain AN1 was isolated from a screening program for nitrile- and amide-hydrolyzing microorganisms in Antarctic desert soil samples. Strain AN1 showed significant 16S rRNA sequence identity to known members ... -
Nodulation, leaf harvesting intensity and interval of the black-eyed bean (BEB) (Vigna unguiculata L. Walpers) cowpea type at the University of Zimbabwe, Harare, Zimbabwe
(Academic Journals, 2012)A glasshouse pot experiment was carried out at the University of Zimbabwe, Crop Science Department in 2006 to 2007 rainy season to determine the effect of leaf harvesting intensity and interval on cowpea biological nitrogen ... -
Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings
(Elsevier, 2012)Sorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular mechanisms ... -
Molecular adaptation mechanisms employed by ethanologenic bacteria in response to lignocellulose-derived inhibitory compounds
(Ivyspring, 2013)Current international interest in finding alternative sources of energy to the diminishing supplies of fossil fuels has encouraged research efforts in improving biofuel production technologies. In countries which lack ... -
Modulation of antioxidant enzyme activities and metabolites ratios by nitric oxide in short-term salt stressed soybean root nodules
(Elsevier, 2013)Several abiotic factors cause molecular damage to plants either directly or through the accumulation of reactive oxygen species such as hydrogen peroxide (H2O2). We investigated if application of nitric oxide (NO) donor ... -
Balancing redox cofactor generation and ATP synthesis: key microaerobic responses in thermophilic fermentations
(Wiley, 2013)Geobacillus thermoglucosidasius is a Grampositive, thermophilic bacterium capable of ethanologenic fermentation of both C5 and C6 sugars and may have possible use for commercial bioethanol production [Tang et al., 2009; ... -
Nitric oxide (NO) regulates the expression of single-domain cystatins in glycine max (soybean)
(Southern Cross Publishing, 2013)Plant cystatins inhibit cysteine proteases and are important in regulating plant development and plant responses to biotic and abiotic stress. Furthermore, nitric oxide plays a signaling role in regulating plant responses ... -
Assessment of temporal and spatial evolution of bacterial communities in a biological sand filter mesocosm treating winery wastewater
(The Society for Applied Microbiology, 2013)AIMS: To assess the impact of winery wastewater (WW) on biological sand filter (BSF) bacterial community structures, and to evaluate whether BSFs can constitute alternative and valuable treatment-processes to remediate WW. ...
