Browsing Research Articles (Medical Bioscience) by Author "Fielding, Burtram C."
Now showing items 21-34 of 34
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MERS-CoV: Understanding the latest human coronavirus threat
Chafekar, Aasiyah; Fielding, Burtram C. (MDPI, 2018)Human coronaviruses cause both upper and lower respiratory tract infections in humans. In 2012, a sixth human coronavirus (hCoV) was isolated from a patient presenting with severe respiratory illness. The 60-year-old man ... -
Molecular detection of tick-borne pathogen diversities in ticks from livestock and reptiles along the shores and adjacent islands of lake Victoria and lake Baringo, Kenya
Omondi, David; Masiga, Daniel K.; Fielding, Burtram C.; Kariuki, Edward; Ajamma, Yvonne Ukamaka (Frontiers Media, 2017)Tick-borne pathogens (TBPs) are responsible for some of the most serious emerging infectious diseases facing sub-Saharan Africa (SSA) and the rest of the world today (1, 2). In Kenya, TBPs (including viral diseases—arboviruses) ... -
Monoclonal Antibodies targeting the HR2 Domain and the region immediately upstream of the HR2 of the S Protein neutralize in Vitro Infection of Severe Acute Respiratory Syndrome Coronavirus
Lip, Kuo-Ming; Shen, Shuo; Yang, Xiaoming; Keng, Choong-Tat; Zhang, Aihua; Oh, Hsueh-Ling Janice; Li, Zhi-Hong; Hwang, Le-Ann; Chou, Chih-Fong; Fielding, Burtram C.; Tan, Timothy H.P.; Mayrhofer, Josef; Falkner, Falko G.; Fu, Jianlin; Lim, Seng Gee; Hong, Wanjin; Tan, Yee-Joo (American Society for Microbiology, 2006)We have previously shown that an Escherichia coli-expressed, denatured spike (S) protein fragment of the severe acute respiratory coronavirus, containing residues 1029 to 1192 which include the heptad repeat 2 (HR2) ... -
The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein
Kumar, Purnima; Gunalan, Vithiagaran; Liu, Boping; Chow, Vincent T.K.; Druce, Julian; Birch, Chris; Catton, Mike; Fielding, Burtram C.; Tan, Yee-Joo; Lal, Sunil K. (Elsevier, 2007)Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The SARS-CoV genome is predicted to contain 14 functional open reading frames (ORFs). The ... -
A novel cell-based binding assay system reconstituting interaction between SARS-CoV S protein and its cellular receptor
Chou, Chih-Fong; Shen, Shuo; Tan, Yee-Joo; Fielding, Burtram C.; Tan, Timothy H.P.; Fu, Jianlin; Xu, Qiurong; Lim, Seng Gee; Hong, Wanjin (Elsevier, 2005)Severe acute respiratory syndrome (SARS), a life-threatening disease, is caused by the newly identified virus SARS coronavirus (SARSCoV). In order to study the spike (S) protein of this highly contagious virus, we established ... -
A Novel Severe Acute Respiratory Syndrome Coronavirus Protein, U274, is transported to the Cell Surface and undergoes Endocytosis
Tan, Yee-Joo; Teng, Eileen; Shen, Shuo; Tan, Timothy H.P.; Goh, Phuay-Yee; Fielding, Burtram C.; Ooi, Eng-Eong; Tan, Hwee-Cheng; Lim, Seng Gee; Hong, Wanjin (American Society for Microbiology, 2004)The severe acute respiratory syndrome coronavirus (SARS-CoV) genome contains open reading frames (ORFs) that encode for several genes that are homologous to proteins found in all known coronaviruses. These are the replicase ... -
Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells
Khan, Sehaam; Fielding, Burtram C.; Tan, Timothy H.P.; Chou, Chih-Fong; Shou, Shen; Lim, Seng Gee; Hong, Wanjin; Tan, Yee-Joo (Elsevier, 2006)The genome of the severe acute respiratory syndrome coronavirus encodes for eight accessory viral proteins with no known homologues in other coronaviruses. One of these is the 3b protein, which is encoded by the second ... -
Overexpression of 7a, a Protein Specifically Encoded by the Severe Acute Respiratory Syndrome Coronavirus, Induces Apoptosis via a Caspase-Dependent Pathway
Tan, Yee-Joo; Fielding, Burtram C.; Goh, Phuay-Yee; Shen, Shuo; Tan, Timothy H.P.; Lim, Seng Gee; Hong, Wanjin (American Society for Microbiology, 2004)Besides genes that are homologous to proteins found in other coronaviruses, the severe acute respiratory syndrome coronavirus genome also contains nine other potential open reading frames. Previously, we have characterized ... -
Profiles of antibody responses against severe acute respiratory syndrome coronavirus recombinant proteins and their potential use as diagnostic markers
Tan, Yee-Joo; Goh, Phuay-Yee; Fielding, Burtram C.; Shen, Shuo; Chou, Chih-Fong; Fu, Jianlin; Leong, Hoe Nam; Leo, Yee Sin; Ooi, Eng-Eong; Ling, Ai Ee; Lim, Seng Gee; Hong, Wanjin (American Society for Microbiology, 2004) -
The role of Severe Acute Respiratory Syndrome (SARS)-Coronavirus Accessory Proteins in Virus Pathogenesis
McBride, Ruth; Fielding, Burtram C. (MDPI, 2012)A respiratory disease caused by a novel coronavirus, termed the severe acute respiratory syndrome coronavirus (SARS-CoV), was first reported in China in late 2002. The subsequent efficient human-to-human transmission of ... -
The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells
Tan, Yee-Joo; Tham, Puay-Yoke; Chan, Daphne Z. L.; Chou, Chih-Fong; Shen, Shuo; Fielding, Burtram C.; Tan, Timothy H.P.; Lim, Seng Gee; Hong, Wanjin (American Society for Microbiology, 2005)Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, ... -
Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT
Fielding, Burtram C.; Gunalan, Vithiagaran; Tan, Timothy H.P.; Chou, Chih-Fong; Shen, Shuo; Khan, Sehaam; Lim, Seng Gee; Hong, Wanjin; Tan, Yee-Joo (Elsevier, 2006)Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing ... -
Understanding human coronavirus HCoV-NL63
Abdul-Rasool, Sahar; Fielding, Burtram C. (Bentham Science, 2010)Even though coronavirus infection of humans is not normally associated with severe diseases, the identification of the coronavirus responsible for the outbreak of severe acute respiratory syndrome showed that highly ... -
The variable N-terminal region of DDX5 contains structural elements and auto-inhibits its interaction with NS5B of hepatitis C virus
Dutta, Sujit; Gupta, Garvita; Choi, Yook-Wah; Kotaka, Masayo; Fielding, Burtram C.; Song, Jianxing; Tan, Yee-Joo (Portland Press, 2012)RNA helicases of the DEAD (Asp-Glu-Ala-Asp)-box family of proteins are involved in many aspects of RNA metabolism from transcription to RNA decay, but most of them have also been shown to be multifunctional. The DEAD-box ...