Browsing Research Articles (Medical Bioscience) by Subject "DNA fragmentation"
Now showing items 1-8 of 8
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Controversy and consensus on indications for sperm dna fragmentation testing in male infertility: a global survey, current guidelines, and expert recommendations
(Korean Society for Sexual Medicine and Andrology, 2023)Purpose: Sperm DNA fragmentation (SDF) testing was recently added to the sixth edition of the World Health Organization laboratory manual for the examination and processing of human semen. Many conditions and risk factors ... -
Critical evaluation of two models of flow cytometers for the assessment of sperm DNA fragmentation: an appeal for performance verification
(Medknow Publications, 2019)Lack of standardized, reproducible protocols and reference values is among the challenges faced when using new or upgraded versions of instruments in reproductive laboratories and flow cytometry. Terminal deoxynucleotidyl ... -
Effect of Cissampelos capensis rhizome extract on human spermatozoa in vitro
(Wiley, 2014)Cissampelos capensis is commonly known by the Afrikaans name ‘dawidjies’ or ‘dawidjieswortel’. C. capensis is the most important and best-known medicinal plant of the family Menispermaceae used by the Khoisan and other ... -
Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa
(Wiley, 2019)The aim of this study was to evaluate motility, viability, DNA integrity and apoptosis of spermatozoa when washed semen samples were kept for up to 12 days at 4–6°C and 25°C. In this experimental study, 26 normozoospermic ... -
The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment
(Elsevier, 2013)Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher ... -
The sixth edition of the WHO manual for human semen analysis: A critical review and SWOT analysis
(MDPI, 2021)Semen analysis is the cornerstone of male fertility evaluation with WHO guidelines providing the basis for procedural standardization and reference values worldwide. The first WHO manual was published in 1980, and five ... -
Smoking‐induced genetic and epigenetic alterations in infertile men
(Wiley, 2018)Male fertility rates have shown a progressive decrease in both developing and industrialised countries in the past 50 years. Clinical and epidemiological studies have demonstrated controversial results about the harmful ... -
TUNEL assay—Standardized method for testing sperm DNA fragmentation
(Blackwell Publishing Ltd, 2021)Sperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor ...