Browsing Research Articles (IMBM) by Title
Now showing items 1-20 of 35
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16 S rDNA primers and the unbiased assessment of thermophile diversity
(Portland Press, 2004)Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA. ‘Universal’ and domain-specific rRNA gene PCR primers have historically been used for the assessment of microbial diversity ... -
Antarctic Dry Valley mineral soils contain unexpectedly high levels of microbial biomass
(Springer Verlag, 2002)We have applied bioluminescent ATP detection methods to microbial enumeration in Antarctic Dry Valley mineral soils, and validated our ATP data by two independent methods. We have demonstrated that ATP measurement is ... -
Biodegradation of high-concentration isopropanol by a solvent-tolerant thermophile, Bacillus pallidus
(Springer Verlag, 2002)The aerobic biodegradation of high-concentration, to 24 g l –1 , 2-propanol (IPA) by a thermophilic isolate ST3, identified as Bacillus pallidus , was successfully carried out for the first time. This solvent-tolerant B. ... -
Characterisation of campylobacter concisus strains from South Africa using amplified fragment length polymorphism (AFLP) profiling and a genomospecies-specific polymerase chain reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical data
(Academic Journals, 2013)Amplified Fragment Length Polymorphism (AFLP) profiling was used to evaluate the distribution of phenotypically indistinguishable, but genetically distinct, among Campylobacter concisus strains from South Africa. A Polymerase ... -
Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids
(Academy of Science of South Africa, 2010)An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the ... -
Cloning, expression and characterization of thermostable YdaP from Bacillus licheniformis 9A
(Acta Biochimica Polonica, 2017)The Bacillus licheniformis ydaP gene encodes for a pyruvate oxidase that catalyses the oxidative decarboxylation of pyruvate to acetate and CO2. The YdaP form of this enzyme was purified about 48.6-folds to homogeneity in ... -
A designed experiments approach to optimizing MALDI-TOF MS spectrum processing parameters enhances detection of antibiotic resistance in Campylobacter jejuni
(Frontiers Media, 2016)MALDI-TOF MS has been utilized as a reliable and rapid tool for microbial fingerprinting at the genus and species levels. Recently, there has been keen interest in using MALDI-TOF MS beyond the genus and species levels to ... -
Dissemination and survival of non-indigenous bacterial genomes in pristine Antarctic environments.
(Springer Verlag, 2005)Continental Antarctic is perceived as a largely pristine environment, although certain localized regions (e.g., parts of the Ross Dependency Dry Valleys) are relatively heavy impacted by human activities. The procedures ... -
Draft Genome Sequence of Gordonia lacunae BS2T
(American Society for Microbiology, 2017)We report here the draft genome sequence of the soil bacterium Gordonia lacunae BS2T ( DSM 45085T JCM 14873T NRRL B-24551T), isolated from an estuary in Plettenberg Bay, South Africa. Analysis of the draft genome ... -
Effect of Fe-Mo promoters on HZSM-5 zeolitecatalyst for 1-hexene aromatization
(Elsevier B.V., 2018-11-15)The promotional effect of Fe-Mo species introduced into HZSM-5 (Zeolyst Int.,SiO2/Al2O3 30) zeolite catalyst by the wetness impregnation method for the 1-hexene aromatiza-tion was investigated. The structure and catalytic ... -
Efficient molecular cloning of environmental DNA from geothermal sediments
(Kluwer Academic Publishers, 2002)An efficient and simple method for constructing an environmental library using mechanically sheared DNA obtained directly from geothermal sediments is presented. The method is based on blunt-end modification of DNA fragments ... -
Engineering pyruvate decarboxylase-mediated ethanol production in the thermophilic host Geobacillus thermoglucosidasius
(Springer Verlag, 2013)This study reports the expression, purification, and kinetic characterization of a pyruvate decarboxylase (PDC) from Gluconobacter oxydans . Kinetic analyses showed the enzyme to have high affinity for pyruvate (120 μM at ... -
High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica
(Springer Verlag, 2003)The microbial diversity in maritime meltwater pond sediments from Bratina Island, Ross Sea, Antarctica was investigated by 16S rDNA-dependent molecular phylogeny. Investigations of the vertical distribution, phylogenetic ... -
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils
(American Society for Microbiology, 2014)The metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities, cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded ... -
Hypolithic and soil microbial community assembly along an aridity gradient in the Namib Desert
(Springer, 2013)The Namib Dessert is considered the oldest desert in the world and hyperarid for the last 5 million years. However, the environmental buffering provided by quartz and other translucent rocks supports extensive hypolithic ... -
International nonproprietary names for monoclonal antibodies: an evolving nomenclature system
(Taylor and Francis, 2022)Appropriate nomenclature for all pharmaceutical substances is important for clinical development, licensing, prescribing, pharmacovigilance, and identification of counterfeits. Nonproprietary names that are unique and ... -
Metagenomics, gene discovery and the ideal biocatalyst
(Portland Press, 2004)With the rapid development of powerful protein evolution and enzyme-screening technologies, there is a growing belief that optimum conditions for biotransformation processes can be established without the constraints of ... -
Micro-Eukaryotic diversity in Hypolithons from Miers Valley, Antarctica
(Multidisciplinary Digital Publishing Institute (MDPI), 2013)The discovery of extensive and complex hypolithic communities in both cold and hot deserts has raised many questions regarding their ecology, biodiversity and relevance in terms of regional productivity. However, most ... -
Molecular characterization of the 16S rRNA Gene of helicobacter fennelliae isolated from stools and blood cultures from paediatric patients in South Africa
(Hindawi, 2011)Forty strains of H. fennelliae collected from paediatric blood and stool samples over an 18 year period at a children's hospital in Cape Town, South Africa, were amplified by PCR of the 16S rRNA. Two distinct genotypes ... -
Multilocus sequence typing methods for the emerging Campylobacter species C. hyointestinalis, C. lanienae, C. sputorum, C. concisus, and C. curvus
(Frontiers Media, 2012)Multilocus sequence typing (MLST) systems have been reported previously for multiple food - and food animal-associated Campylobacter species (e.g., C. jejuni, C. coli, C. lari, and C. fetus) to both differentiate strains ...
