dc.contributor.author | Owino, Joseph H.O. | |
dc.contributor.author | Arotiba, Omotayo A. | |
dc.contributor.author | Hendricks, Nicolette | |
dc.contributor.author | Songa, Everlyne | |
dc.contributor.author | Jahed, Nazeem | |
dc.contributor.author | Waryo, Tesfaye T. | |
dc.contributor.author | Ngece, Rachel F. | |
dc.contributor.author | Baker, Priscilla | |
dc.contributor.author | Iwuoha, Emmanuel I. | |
dc.date.accessioned | 2016-12-21T12:55:16Z | |
dc.date.available | 2016-12-21T12:55:16Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Owino, J.H.O. et al. (2008). Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of Aflatoxin B1. Sensors, 8(12), 8262-8274 | en_US |
dc.identifier.issn | 1424-8220 | |
dc.identifier.uri | http://hdl.handle.net/10566/2475 | |
dc.identifier.uri | http://dx.doi.org/10.3390/s8128262 | |
dc.description.abstract | An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the
immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a
polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE).
The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP),
in order to prevent non-specific binding of the immunosensors with ions in the test
solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as
indicated by a cyclic voltammetric (CV) peak separation (ΔEp) value of 62 mV. The
experimental procedure for the detection of AFB1 involved the setting up of a competition
between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free
anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor’s differential pulse
voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1
increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of
detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need
for enzyme-labeled secondary antibodies normally used in conventional ELISA–based
immunosensors. | en_US |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.rights | All articles published by MDPI are made immediately available worldwide under an open access license. | |
dc.subject | Immunosensor | en_US |
dc.subject | Gold nanoparticles | en_US |
dc.subject | Aflatoxin B1 | en_US |
dc.subject | Polythionine | en_US |
dc.subject | Horseradish peroxidise (HRP) | en_US |
dc.title | Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of Aflatoxin B1 | en_US |
dc.type | Article | en_US |