Browsing Biotechnology by Title
Now showing items 110-129 of 215
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Identification and characterization of a heat-inducible Hsp70 gene from Sorghum bicolor which confers tolerance to thermal stress
(Springer, 2015)This study describes the first detailed molecular characterization of the heat shock protein 70 (Hsp70) gene from Sorghum bicolor, MN1618 designated as SbHsp70-1. The full-length cDNA of SbHsp70-1 consists of 2524 bp ... -
Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings
(Elsevier, 2012)Sorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular mechanisms ... -
Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from Antarctic desert soil
(American Society for Microbiology, 2009)The cold deserts of the McMurdo Dry Valleys, South Victoria Land, Eastern Antarctica, are widely acknowledged as having the harshest soil environments on Earth (6, 8, 26). Despite the apparent hostility of the environment, ... -
Identification of a novel protein with guanylyl cyclase activity in Arabidopsis thaliana
(Elsevier, 2003)Guanylyl cyclases (GCs) catalyze the formation of the second messenger guanosine 3 ,5 -cyclic monophos- phate (cGMP) from guanosine 5 -triphosphate (GTP). While many cGMP-mediated processes in plants have been reported, ... -
Identification of biomarkers for the accurate and sensitive diagnosis of three bacterial pneumonia pathogens using in silico approaches
(Springer Nature, 2020): Pneumonia ranks as one of the main infectious sources of mortality among kids under 5 years of age, killing 2500 a day; late research has additionally demonstrated that mortality is higher in the elderly. A few ... -
Identification of superior cellulase secretion phenotypes in haploids derived from natural Saccharomyces cerevisiae isolates
(Oxford University Press, 2019)The yeast Saccharomyces cerevisiae is considered an important host for consolidated bioprocessing and the production of high titres of recombinant cellulases is required for efficient hydrolysis of lignocellulosic substrates ... -
Immunoinformatics design of a novel epitope-based vaccine candidate against dengue virus
(Springer, 2021-10)Dengue poses a global health threat, which will persist without therapeutic intervention. Immunity induced by exposure to one serotype does not confer long-term protection against secondary infection with other serotypes ... -
Impact of metagenomic DNA extraction procedures on the identifiable endophytic bacterial diversity in Sorghum bicolor (L. Moench)
(Elsevier, 2015)Culture-independent studies rely on the quantity and quality of the extracted environmental metagenomic DNA (mDNA). To fully access the plant tissue microbiome, the extracted plant mDNA should allow optimal PCR applications ... -
Improved cellulase expression in diploid yeast strains enhanced consolidated bioprocessing of pretreated corn residues
(ScienceDirect, 2019)In an effort to find a suitable genetic background for efficient cellulolytic secretion, genetically diverse strains were transformed to produce core fungal cellulases namely, β-glucosidase (BGLI), endoglucanase (EGII) and ... -
Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain
(Springer, 2021)The expression of functional proteins on the cell surface using glycosylphosphatidylinositol (GPI)-anchoring technology is a promising approach for constructing yeast cells with special functions. The functionality of ... -
In silico identification of micrornas as candidate Colorectal cancer biomarkers
(Sage Journals, 2019)The involvement of microRNA in cancers plays a significant role in their pathogenesis. Specific expressions of these non-coding RNAs also serve as biomarkers for early colorectal cancer diagnosis, but their laboratory/molecular ... -
In silico prediction of new antimicrobial peptides and proteins as druggable targets towards alternative anti-schistosomal therapy
(Elsevier, 2021)Schistosomiasis is a debilitating disease caused by a parasitic flatworm found in fresh- water. With the exponential increase in prevalence, Praziquantel (PZQ) remains the only effective treatment drug, however, resistance ... -
In vitro Antidiabetic and Antioxidant effects of different extracts of Catharanthus roseus and its indole alkaloid, vindoline
(MPDI, 2020)The Catharanthus roseus plant has been used traditionally to treat diabetes mellitus. Scientific evidence supporting the antidiabetic effects of this plant’s active ingredient-vindoline has not been fully evaluated. In ... -
In vitro evaluation of the anti-diabetic potential of aqueous acetone Helichrysum petiolare extract (AAHPE) with molecular docking relevance in diabetes mellitus
(MDPI, 2021-12)Diabetes mellitus (DM) is a chronic metabolic condition that can lead to significant complications and a high fatality rate worldwide. Efforts are ramping up to find and develop novel α-glucosidase and α-amylase inhibitors ... -
An in vitro study on the combination effect of metformin and n-acetyl cysteine against hyperglycaemia-induced cardiac damage
(MDPI - Publisher of Open Access Journals, 2019)Chronic hyperglycaemia is a major risk factor for diabetes-induced cardiovascular dysfunction. In a hyperglycaemic state, excess production of reactive oxygen species (ROS), coupled with decreased levels of glutathione, ... -
In-vitro cytotoxicity, anti-bacterial and anti-inflammatory activities of ecklonia radiataand jania verrucosafrom Eastern Cape, South Africa
(University of Benin, 2022)Seaweeds are marine floras constituting in part to over 90% of the oceanic biomass. Despite the enrichments of seaweeds, their potentials are largely unexplored. Hence, this study evaluated the phytochemical screening, ... -
Increasing extracellular cellulase activity of the recombinant Saccharomyces cerevisiae by engineering cell wall-related proteins for improved consolidated processing of carbon neutral lignocellulosic biomass
(Elsevier, 2022)Sustainable bioproduction using carbon neutral feedstocks, especially lignocellulosic biomass, has attracted increasing attention due to concern over climate change and carbon reduction. Consolidated bioprocessing (CBP) of ... -
Indigenous African cereal crops can contribute to mitigation of the impact of climate change on food security
(Arid land institute, 2021)Zea mays L. (maize) is one of the top three cultivated cereals globally, along with wheat and rice. The United States, China, and Brazil are among the largest producers of maize, producing approximately 79% of the world’s ... -
Inhibition of bacteria associated with wound infection by biocompatible green synthesized gold nanoparticles from South African plant extracts
(MDPI, 2017)Unlike conventional physical and chemical methods, the biogenic synthesis of gold nanoparticles (GNPs) is considered a green and non-toxic approach to produce biocompatible GNPs that can be utilized in various biomedical ... -
Inhibition of NOS- like activity in maize alters the expression of genes involved in H2O2 scavenging and glycine betaine biosynthesis
(Nature Publishing Group, 2018)Nitric oxide synthase-like activity contributes to the production of nitric oxide in plants, which controls plant responses to stress. This study investigates if changes in ascorbate peroxidase enzymatic activity and ...