Biotechnology: Recent submissions
Now showing items 21-40 of 219
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Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form
(Wiley, 2007)Nitrilases convert nitriles to the corresponding carboxylic acids and ammonia. The nitrilase from Rhodococcus rhodochrous J1 is known to be inactive as a dimer but to become active on oligomerization. The recombinant enzyme ... -
Characterization and expression analysis of heme oxygenase genes from sorghum bicolor
(SAGE Publications, 2019)Heme oxygenases (HOs) have a major role in phytochrome chromophore biosynthesis, and chromophores in turn have antioxidant properties. Plant heme oxygenases are divided into the HO1 sub-family comprising HO1, HO3, and ... -
Reports of Plant-Derived Nanoparticles for Prostate Cancer Therapy
(Plants, 2023)Background: Plants have demonstrated potential in providing various types of phytomedicines with chemopreventive properties that can combat prostate cancer. However, despite their promising in vitro activity, the incorporation ... -
Ozone and nitric oxide induce cGMP-dependent and independent transcription of defence genes in tobacco
(Wiley, 2009)Here, we analyse the temporal signatures of ozone (O3)-induced hydrogen peroxide (H2O2) and nitric oxide (NO) and the role of the second messenger guanosine 3′,5′-cyclic monophosphate (cGMP) in transcriptional changes ... -
Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from Antarctic desert soil
(American Society for Microbiology, 2009)The cold deserts of the McMurdo Dry Valleys, South Victoria Land, Eastern Antarctica, are widely acknowledged as having the harshest soil environments on Earth (6, 8, 26). Despite the apparent hostility of the environment, ... -
Reports of plant-derived nanoparticles for prostate cancer therapy
(MDPI, 2023)Plants have demonstrated potential in providing various types of phytomedicines with chemopreventive properties that can combat prostate cancer. However, despite their promising in vitro activity, the incorporation of these ... -
Reports of plant-derived nanoparticles for prostate cancer therapy
(MDPI, 2023)Plants have demonstrated potential in providing various types of phytomedicines with chemopreventive properties that can combat prostate cancer. However, despite their promising in vitro activity, the incorporation of ... -
Hypoglycaemic activity of biosynthesized copper oxide nanoparticles in alloxan-induced diabetic Wister rats
(Wiley, 2023)Diabetes mellitus (DM) is a metabolic disorder that affects the body's ability to produce or use insulin. This study evaluated the hypoglycaemic activity of biosynthesized copper oxide nanoparticles (CuO-NPs) ... -
Proteomic analysis of grape berry cell cultures reveals that developmentally regulated ripening related processes can be studied using cultured cells
(Public Library of Science, 2011)This work describes a proteomics profiling method, optimized and applied to berry cell suspensions to evaluate organ-specific cultures as a platform to study grape berry ripening. Variations in berry ripening within a ... -
Development of infectious transcripts and genome manipulation of black queen-cell virus of honey bees
(Microbiology Society, 2002)The South African isolate of Black queen-cell virus (BQCV), a honey bee virus, was previously found to have an 8550 nucleotide genome excluding the poly(A) tail. Its genome contained two ORFs, a 5«-proximal ORF encoding ... -
Variability in Responses to Phoma medicaginis Infection in a Tunisian Collection of Three Annual Medicago Species
(Korean Society of Plant Pathology, 2023)Spring black stem and leaf spot, caused by Phoma medicaginis, is an issue in annual Medicago species. Therefore, in this study, we analyzed the response to P. medicaginis infection in a collection of 46 lines of three ... -
Green synthesis of iron oxide (hematite) nanoparticles and their influence on sorghum bicolor growth under drought stress
(MDPI, 2023)Drought is a major abiotic stress that confronts plant growth and productivity, thus compromising food security. Plants use physiological and biochemical mechanisms to cope with drought stress, but at the expense of ... -
Genetic heritage of the Baphuthi highlights an over-ethnicized notion of “Bushman” in the Maloti-Drakensberg, southern Africa
(Cell Press, 2023)Using contemporary people as proxies for ancient communities is a contentious but necessary practice in anthropology. In southern Africa, the distinction between the Cape KhoeSan and eastern KhoeSan remains unclear, as ... -
Comparative venom toxin analyses of Nigerian viperidae and elapidae snakes
(Elsevier, 2023)Envenoming by snakebite is a serious health problem that maims and kills a large number of people, primarily in rural areas of developing African countries. The first comparative venom proteomic analyses of four snakes ... -
Anticancer and drug-sensitizing activities of gold nanoparticles synthesized from cyclopia genistoides (honeybush) extracts
(MDPI, 2023)Synthesis of gold nanoparticles (AuNPs) using phytochemicals has become tremendously prominent in biomedical applications because of its enhanced bioactivity and biocompatibility. In this study, water extracts from the ... -
Prospects of using gum Arabic silver nanoparticles in toothpaste to prevent dental caries
(MDPI, 2023)There is growing interest in the use of green synthesized silver nanoparticles (AgNPs) to control and prevent dental diseases. The incorporation of green synthesized AgNPs into dentifrices to reduce pathogenic oral ... -
A guide to molecular characterization of genotype ii African swine fever virus: Essential and alternative genome markers
(MDPI, 2023)African swine fever is a contagious viral disease that has been spreading through Europe and Asia since its initial report from Georgia in 2007. Due to the large genome size of the causative agent, the African swine fever ... -
In-vitro cytotoxicity, anti-bacterial and anti-inflammatory activities of ecklonia radiataand jania verrucosafrom Eastern Cape, South Africa
(University of Benin, 2022)Seaweeds are marine floras constituting in part to over 90% of the oceanic biomass. Despite the enrichments of seaweeds, their potentials are largely unexplored. Hence, this study evaluated the phytochemical screening, ... -
Chitosan-coated liposomes of Carissa spinarum extract: synthesis, analysis and antipneumococcal potency
(ICE Publishing, 2023)In the present study, a chitosan-coatedCarissa spinarum-polyphenol-loaded liposome (LipCsP-chitosan) nanocarrierwas fabricated for the delivery ofC. spinarumpolyphenols (CsPs) to improve the bioavailability ... -
Using multiplex amplicon pcr technology to efficiently and timely generate rift valley fever virus sequence data for genomic surveillance
(MDPI, 2023)Rift Valley fever (RVF) is a febrile vector-borne disease endemic in Africa and continues to spread in new territories. It is a climate-sensitive disease mostly triggered by abnormal rainfall patterns. The disease is ...