Browsing Research Articles (SANBI) by Author "Cloete, Ruben"
Now showing items 1-7 of 7
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Analyses of HIV-1 integrase sequences prior to South African national HIV-treatment program and available of integrase inhibitors in Cape Town, South Africa
Brado, Dominik; Obasa, Adetayo Emmanuel; Ikomey, George Mondinde; Cloete, Ruben; Singh, Kamalendra; Engelbrecht, Susan; Neogi, Ujjwal; Jacobs, Graeme Brendon (Nature Publishing Group, 2018)HIV-Integrase (IN) has proven to be a viable target for highly specific HIV-1 therapy. We aimed to characterize the HIV-1 IN gene in a South African context and identify resistance-associated mutations (RAMs) against ... -
Designing a course model for distance-based online bioinformatics training in Africa: the H3ABioNet experience
Gurwitz, Kim T.; Aron, Shaun; Entfellner, Jean-Baka Domelevo; Saunders, Colleen J.; Cloete, Ruben (Public Library of Science, 2017)Africa is not unique in its need for basic bioinformatics training for individuals from a diverse range of academic backgrounds. However, particular logistical challenges in Africa, most notably access to bioinformatics ... -
Drug resistance mutations against protease, reverse transcriptase and integrase inhibitors in people living with HIV-1 receiving boosted protease inhibitors in South Africa
Cloete, Ruben; Obasa, Adetayo Emmanuel; Mikasi, Sello Given (Frontiers Media S.A., 2020)The South African national combination antiretroviral therapy (cART) roll-out program started in 2006, with over 4.4 million people accessing treatment since it was first introduced. HIV-1 drug resistance can hamper the ... -
Genome sequencing of a severe acute respiratory syndrome Coronavirus 2 isolate obtained from a South African patient with Coronavirus disease 2019
Cloete, Ruben; Allam, Mushal M.; Khumalo, Zamantungwa T.H. (American Society for Microbiology, 2020)As a contribution to the global efforts to track and trace the ongoing coronavirus pandemic, here we present the sequence, phylogenetic analysis, and modeling of nonsynonymous mutations for a severe acute respiratory ... -
HIV-1 diversity and the implementation of integrase strand-transfer inhibitors as part of combination antiretroviral therapy
Cloete, Ruben; Mikasi, Sello Given; Ikomey, George Mondinde (SAMA, 2020): The integrase (IN) strand-transfer inhibitor (InSTI) dolutegravir (DTG) is now recommended by the World Health Organization as part of salvage and/or first-line combination antiretroviral therapy (cART).[1] DTG has a ... -
In silico repurposing of a Novobiocin derivative for activity against latency associated Mycobacterium tuberculosis drug target nicotinate-nucleotide adenylyl transferase (Rv2421c)
Cloete, Ruben; Shahbaaz, Mohd; Christoffels, Alan (Public Library of Science, 2021)Nicotinamide-nucleotide adenylyl transferase (Rv2421c) was selected as a potential drug target, because it has been shown, in vitro, to be essential for Mycobacterium tuberculosis growth. It is conserved between mycobacterium ... -
Structure based identification of novel inhibitors against ATP synthase of Mycobacterium tuberculosis: A combined in silico and in vitro study
Cloete, Ruben; Grobbelaar, Melanie; Sampson, Samantha; Christoffels, Alan (International Journal of Biological Macromolecules, 2019)The shortcomings of conventional tuberculosis treatments resulting from the development of drug resistance in Mycobacteriumtuberculosis drive a need for the formulation of novel therapeutic agents. The diarylquinoline ...