Browsing by Author "Henkel, Ralf"
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Association between promoter methylation of MLH1 and MSH2 and reactive oxygen species in oligozoospermic men—A pilot study
Gunes, S.; Agarwal, A.; Henkel, Ralf; Sharma, Rajan; Esteves, S.C.; Aljowair, A.; Emirzeoglu, D.; Alkhani, A.; Pelegrini, L.; Joumah, A.; Sabanegh, E.; Mahmutoglu, A.M. (Wiley, 2017)MLH1 and MSH2 are important genes for DNA mismatch repair and crossing over during meiosis and are implicated in male infertility. Therefore, the methylation patterns of the DNA mismatch repair genes MLH1 and MSH2 in ... -
Automation of human semen analysis using a novel artificial intelligence optical microscopic technology
Henkel, Ralf (Wiley Online Library, 2019)Current semen analysis still commonly depends on a manual microscopy method in clinical laboratories worldwide. However, some of the major disadvantages of this technique are that it is labour‐intensive, subjective, ... -
Bibliometrics: tracking research impact by selecting the appropriate metrics
Henkel, Ralf (Medknow Publications, 2016)Traditionally, the success of a researcher is assessed by the number of publications he or she publishes in peer-reviewed, indexed, high impact journals. This essential yardstick, often referred to as the impact of a ... -
Calibration of redox potential in sperm wash media and evaluation of oxidation–reduction potential values in various assisted reproductive technology culture media using MiOXSYS system
Panner Selvam, M. K.; Henkel, Ralf; Sharma, Rajan; Agarwal, A. (Wiley, 2018)Oxidation–reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to ... -
Carica papaya seed extract slows human sperm
Ghaffarilaleh, Vahid; Fisher, David; Henkel, Ralf (Elsevier, 2019)Ethnopharmacological relevance: Traditional healers use Carica papaya seeds as a remedy for diseases and as a contraceptive for men and abortion in women. Material and methods: Semen samples from 35 healthy men were allowed ... -
Controversy and consensus on indications for sperm dna fragmentation testing in male infertility: a global survey, current guidelines, and expert recommendations
Henkel, Ralf; Agarwal, Ashok; Farkouh, Ala'a (Korean Society for Sexual Medicine and Andrology, 2023)Purpose: Sperm DNA fragmentation (SDF) testing was recently added to the sixth edition of the World Health Organization laboratory manual for the examination and processing of human semen. Many conditions and risk factors ... -
Critical evaluation of two models of flow cytometers for the assessment of sperm DNA fragmentation: an appeal for performance verification
Sharma, Rakesh; Gupta, Sajal; Henkel, Ralf (Medknow Publications, 2019)Lack of standardized, reproducible protocols and reference values is among the challenges faced when using new or upgraded versions of instruments in reproductive laboratories and flow cytometry. Terminal deoxynucleotidyl ... -
Determination of seminal oxidation–reduction potential (ORP) as an easy and cost-effective clinical marker of male infertility
Agarwal, A.; Henkel, Ralf; Sharma, Rajan; Tadros, N.N.; Sabanegh, E. (Wiley, 2017)Oxidative stress (OS) is an important contributing factor to male infertility. While previous methods to measure seminal OS are time-consuming and limited to the use of freshly produced semen, oxidation reduction potential ... -
Editorial commentary on draft of world health organization sixth edition laboratory manual for the examination and processing of human semen
Kandil, Hussein; Agarwal, Ashok; Henkel, Ralf (Korean Society for Sexual Medicine and Andrology, 2021)Semen examination is the cornerstone of the evaluation of male fertility potential. Despite its apparent simplicity, it is a complex series of assessments with highly variable results that are subject to interpretation. ... -
Effect of Cissampelos capensis rhizome extract on human spermatozoa in vitro
Shalaweh, Salem; Henkel, Ralf; Erasmus, N.; Weitz, Frans (Wiley, 2014)Cissampelos capensis is commonly known by the Afrikaans name ‘dawidjies’ or ‘dawidjieswortel’. C. capensis is the most important and best-known medicinal plant of the family Menispermaceae used by the Khoisan and other ... -
The effect of Nigella sativa oil and metformin on male seminal parameters and testosterone in Wistar rats exposed to an obesogenic diet
Leisegang, Kristian; Almaghrawi, Walid; Henkel, Ralf (Elsevier, 2021)Obesity is a significant global health and socio-economic challenge, and considered an important risk factor for poor health outcomes including male reproductive dysfunction and infertility. As excess adiposity causes ... -
The effect of Nigella sativa oil and metformin on male seminal parameters and testosterone in Wistar rats exposed to an obesogenic diet
Leisegang, Kristian; Almaghrawi, Walid; Henkel, Ralf (Elsevier, 2020)Obesity is a significant global health and socio-economic challenge, and considered an important risk factor for poor health outcomes including male reproductive dysfunction and infertility. As excess adiposity causes ... -
The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
Khalafalla, Kareim; Majzoub, Ahmad; Henkel, Ralf (Wiley, 2021)Our study objective was to assess the effect of various sperm DNA fragmentation levels on clinical intracytoplasmic sperm injection outcome. This retrospective study included 392 patients who underwent ICSI and performed ... -
Effect of the metabolic syndrome on male reproductive function: a case-controlled pilot study
Leisegang, Kristian; Bouic, Patrick J.D.; Henkel, Ralf (Wiley, 2014)The metabolic syndrome (MetS) is a constellation of various risk factors. This study aimed to investigate the effect of MetS on testosterone and progesterone, and semen parameters, in a case-controlled pilot study. Male ... -
Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa
Iranpour, Farhad Golshan; Nateghian, Zohre; Henkel, Ralf (Wiley, 2019)The aim of this study was to evaluate motility, viability, DNA integrity and apoptosis of spermatozoa when washed semen samples were kept for up to 12 days at 4–6°C and 25°C. In this experimental study, 26 normozoospermic ... -
Endocrine contribution to the sexual dysfunction in patients with advanced chronic kidney disease and the role of hyperprolactinemia
Elbardisi, Haitham; Majzoub, Ahmad; Daniel, Christiana; Al, Fadwa Al; Henkel, Ralf (John Wiley and Sons Inc, 2021)In this study, we investigated the prevalence of sexual dysfunction among males with advanced chronic kidney disease and the effect of treating hyperprolactinemia among these patients. In this prospective study, patients ... -
Evaluation of reference values of standard semen parameters in fertile Egyptian men
Zedan, H.; Ismail, S.; Gomaa, A.; Saleh, R.; Henkel, Ralf; Agarwal, A. (Wiley, 2018)The reference values of human semen, published in the WHO’s latest edition in 2010, were lower than those previously reported. The objective of this study was to evaluate reference values of standard semen parameters in ... -
The excessive use of antioxidant therapy: A possible cause of male infertility?
Henkel, Ralf; Sandhu, Inderpreet Singh; Agarwal, Ashok (Wiley, 2018)Reactive oxygen species and oxidative stress are closely associated with various pathologies such as neurodegenerative diseases, ageing and male infertility. Hence, antioxidants such as vitamin C, vitamin E, N‐acetyl ... -
Green nanotechnology as an innovative drug delivery approach for typha capensis and naringenin—new class of phytochemical embedded biocompatible gold nanoparticles in prostate cancer therap
Henkel, Ralf; Pearce, Keenau (Journal of Drug Delivery Science and Technology, 2022)Naringenin, a flavone with a growing body of evidence as an anti-cancer agent, is found in Typha capensis, an indigenous South African plant commonly used in traditional medicine. However, despite favourable in vitro ... -
Green tea (Camellia sinensis) aqueous extract improved human spermatozoa functions in vitro
Henkel, Ralf (ResearchGate, 2022)Background: Idiopathic causes of infertility is associated with oxidative stress. Antioxidants are known to scavenge the excessive production of reactive oxygen species (ROS). Green tea (Camellia sinensis) contains polyphenols ...