Library Portal | UWC Portal
    • Login
    Contact Us | Quick Submission Guide | About Us | FAQs | Login
    View Item 
    •   Repository Home
    • Faculty of Natural Sciences
    • Medical Bioscience
    • Conference Papers (Medical Bioscience)
    • View Item
    •   Repository Home
    • Faculty of Natural Sciences
    • Medical Bioscience
    • Conference Papers (Medical Bioscience)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Proteomics of drug-resistant HIV-associated candidiasis

    Thumbnail
    View/Open
    ASM-ISC 2015 Poster.pdf (318.3Kb)
    Date
    2015
    Author
    Abrantes, Pedro Miguel dos Santos
    Bouic, Patrick J.D.
    Africa, Charlene W.J.
    Metadata
    Show full item record
    Abstract
    Candidiasis and HIV co-infection may cause increased patient morbidity and mortality due to oropharyngeal or systemic dissemination. Limited information exists on the prevalence, antifungal susceptibility profiles and drug resistance mechanisms of Candida species on the African continent, the highest HIV-affected region globally and home to new and emerging drug resistant Candida species. Candida species isolated from the oral mucosa of HIV-positive African patients were found to be resistant to many of the antifungals routinely used in HIV-associated candidiasis. Candida cell membrane fractions were examined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and high performance liquid chromatography mass spectrometry (HPLC/MS) in order to elucidate the cell membrane proteins specifically expressed by antifungal drug resistant isolates. SDS-PAGE and HPLC/MS allowed for the identification of multi-drug resistance efflux transporter CDR2 proteins and the elucidation of Candida colonization mechanisms and pH-responsive proteins, with significant associations observed between specific drug resistance and the duration of antiretroviral (ARV) therapy. This study provided useful information on the mechanisms of antifungal resistance in Candida species. It also formed the basis for further studies to address the transfer of resistance between Candida species in an oral microbial biofilm.
    URI
    http://hdl.handle.net/10566/1690
    Collections
    • Conference Papers (Medical Bioscience) [4]

    DSpace 5.5 | Ubuntu 14.04 | Copyright © University of the Western Cape
    Contact Us | Send Feedback
    Theme by 
    @mire NV
     

     

    Browse

    All of RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    DSpace 5.5 | Ubuntu 14.04 | Copyright © University of the Western Cape
    Contact Us | Send Feedback
    Theme by 
    @mire NV