One-pot synthesis, characterisation and biological activities of gold nanoparticles prepared using aqueous seed extract of Garcinia kola
Date
2023-02Author
Sibuyi, Nicole R.S.
Fadaka, Adewale O.
Anadozie, Scholastica O.
Adewale, Olusola B.
Isitua, Chinwe C.
Davids, Hajierah
Roux, Saartjie
Metadata
Show full item recordAbstract
Recently, biogenic synthesis of gold nanoparticles (AuNPs) has become a focus area in cancer research owing to
the eco-friendliness and cost effectiveness of the synthetic method. In this study, aqueous extract of Garcinia kola
seed (AEGKs) was used for the bio-reduction of Au3+ to Au0. The synthesised AEGKs-AuNPs was characterised by ultraviolet-visible (UV-Vis) spectroscopy, dynamic light scattering (DLS), high-resolution transmission electron microscopy (HRTEM) and Fourier transform-infrared (FT-IR) spectroscopy. The in vitro antioxidant activity of the AEGKs and AEGKs-AuNPs was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability and ferric reducing antioxidant power assays. The AEGKs-AuNPs showed an absorption maximum at 512 nm, and the
HRTEM images revealed mostly, spherical-shaped AuNPs in the size range of 2–17 nm. The FT-IR spectroscopy revealed that polyphenolic compounds and proteins were predominant, and responsible for the reduction and capping of the AuNPs. The AEGKs-AuNPs showed concentration dependent antioxidant activities, while dose dependent in vitro anti-cancer activity of the AEGKs-AuNPs was demonstrated against lungs, prostrate, human cervical and human colon cancer cells, using the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyltetrazolium bromide tetrazolium reduction (MTT) assay. The antioxidant and anti-cancer activities of the AEGKs-AuNPs could be attributed to the presence of phytochemicals and physicochemical properties of the AuNPs.