| dc.contributor.author | Iranpour, Farhad Golshan | |
| dc.contributor.author | Nateghian, Zohre | |
| dc.contributor.author | Henkel, Ralf | |
| dc.date.accessioned | 2023-06-07T07:36:05Z | |
| dc.date.available | 2023-06-07T07:36:05Z | |
| dc.date.issued | 2019 | |
| dc.identifier.citation | Iranpour, F. G. et al. (2019). Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa. Andrologia, 52 (2). https://doi.org/10.1111/and.13485 | en_US |
| dc.identifier.issn | 1439-0272 | |
| dc.identifier.uri | https://doi.org/10.1111/and.13485 | |
| dc.identifier.uri | http://hdl.handle.net/10566/9049 | |
| dc.description.abstract | The aim of this study was to evaluate motility, viability, DNA integrity and apoptosis of
spermatozoa when washed semen samples were kept for up to 12 days at 4–6°C and
25°C. In this experimental study, 26 normozoospermic semen samples were washed
twice in Modified Ham's F10 and resuspended in IVF fertilisation medium. Half of the
specimens were stored at 4–6°C, and the other half was kept at 25°C for 12 days. The
proportions of viable, motile, spermatozoa with double-stranded DNA and apoptotic
spermatozoa were examined during storage time. Apoptosis was measured using annexin
V-PI staining followed by flow cytometry. Results showed that sperm motility
and viability decreased during 12 days of sample storage (p < .001). There was no
significant difference between the two temperatures in terms of motility and viability
for up to 2 days (p < .05). | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Wiley | en_US |
| dc.subject | Bioscience | en_US |
| dc.subject | DNA fragmentation | en_US |
| dc.subject | Sperm motility | en_US |
| dc.subject | Human genetics | en_US |
| dc.subject | Medical Sciences | en_US |
| dc.title | Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa | en_US |
| dc.type | Article | en_US |
