Browsing Medical Bioscience by Author "Fielding, Burtram C."
Now showing items 1-20 of 34
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Acute toxicity studies of the South African medicinal plant Galenia africana
Ng’uni, Tiza; Klaasen, Jeremy A.; Fielding, Burtram C. (Elsevier, 2018)Background: Medicinal plants are used by a large proportion of the global population as complementary and alternative medicines. However, little is known about their toxicity. G. africana has been used to treat wounds, coughs ... -
Additive antibacterial activity of naringenin and antibiotic combinations against multidrug resistant Staphylococcus aureus
Ng'uni, Tiza; Mothlalamme, Thato; Daniels, Raymond; Klaasen, Jeremy; Fielding, Burtram C. (Academic Journals, 2015)Methicillin-resistant Staphylococcus aureus has been causing numerous problems in the health care sector. This is mainly due to its ability to develop resistance to a number of antibiotics used to treat staphylococcal ... -
Amino acids 1055 to 1192 in the S2 Region of severe acute respiratory syndrome Coronavirus S Protein induce neutralizing antibodies: Implications for the development of vaccines and antiviral agents
Keng, Choong-Tat; Zhang, Aihua; Shen, Shuo; Lip, Kuo-Ming; Fielding, Burtram C.; Tan, Timothy H.P.; Chou, Chih-Fong; Loh, Chay Boon; Wang, Sifang; Fu, Jianlin; Yang, Xiaoming; Lim, Seng Gee; Hong, Wanjin; Tan, Yee-Joo (American Society for Microbiology, 2005)The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) interacts with cellular receptors to mediate membrane fusion, allowing viral entry into host cells; hence it is recognized as the primary ... -
Antimicrobial-resistant Klebsiella species isolated from free-range chicken samples in an informal settlement
Fielding, Burtram C.; Mnabisa, Amanda; Gouws, Pieter A.; Morris, Thureyah (Termedia Publishing House, 2012)Sub-therapeutic doses of antimicrobial agents are administered routinely to poultry to aid growth and to prevent disease, with prolonged exposure often resulting in bacterial resistance. Crossover of antibiotic resistant ... -
Cellular Characterization of SARS Coronavirus Nucleocapsid
Goh, Phuay-Yee; Choi, Yook-Wah; Shen, Shuo; Tan, Yee-Joo; Fielding, Burtram C.; Tan, Timothy H.P.; O, Eng-Eong; Lim, Seng Gee; Hong, Wanjin (Leibniz Research Centre for Working Environment and Human Factors, 2004)The Severe and Acute Respiratory Syndrome coronavirus (SARS CoV) is a newly-emerged virus that caused an outbreak of atypical pneumonia in the winter of 2002-2003. Polyclonal antibodies raised against the nucleocapsid (N) ... -
Characterisation of human coronavirus-NL63 nucleocapsid protein
Berry, Michael; Manasse, Taryn-Lee; Tan, Yee-Joo; Fielding, Burtram C. (Academic Journals, 2012)Coronavirus N is a multifunctional protein that plays an essential role in enhancing the efficiency of virus transcription and assembly. This manuscript reports the analysis of HCoV-NL63 N protein by comparing the amino ... -
The characterization and phylogenetic relationship of the trichoplusia ni single capsid nuclear polyhedrosis virus polyhedrin gene
Fielding, Burtram C.; Davison, Sean (Kluwer Academic Publishers, 1999)The polyhedrin gene (polh) was identified from the Trichoplusia ni (Tni) single capsid nuclear polyhedrosis virus (SNPV). An EcoRI fragment containing the truncated polyhedrin gene was detected by hybridization with an ... -
Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus
Fielding, Burtram C.; Tan, Yee-Joo; Shen, Shuo; Tan, Timothy H.P.; Ooi, Eng-Eong; Lim, Seng Gee; Hong, Wanjin; Goh, Phuay-Yee (American Society for Microbiology, 2004)A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and structural proteins. Additionally, ... -
Comparative analysis of human coronavirus-NL63 ORF3 protein homologues
Fielding, Burtram C.; Suliman, Tasnim (Academic Journals, 2009)It has been reported in some studies that the newly discovered human coronavirus NL-63 (HCoVNL63) is one of the most common coronaviruses associated with acute respiratory infections. HCoVNL63 was first isolated in 2004 ... -
The Coronavirus Nucleocapsid is a Multifunctional Protein
McBride, Ruth; van Zyl, Marjorie; Fielding, Burtram C. (MDPI, 2014)The coronavirus nucleocapsid (N) is a structural protein that forms complexes with genomic RNA, interacts with the viral membrane protein during virion assembly and plays a critical role in enhancing the efficiency of virus ... -
Expression, purification and preliminary crystallographic analysis of recombinant human DEAD-box polypeptide 5
Choi, Yook-Wah; Dutta, Sujit; Fielding, Burtram C.; Tan, Yee-Joo (International Union of Crystallography, 2010)The DEAD-box RNA helicase DDX5 is involved in many aspects of RNA processing and has been implicated in a number of cellular processes involving alteration of RNA secondary structure. The N-terminal region of DDX5, ... -
The flexible, extended coil of the pdz-binding motif of the three deadly human coronavirus e proteins plays a role in pathogenicity
Schoeman, Dewald; Cloete, Ruben; Fielding, Burtram C. (MDPI, 2022)The less virulent human (h) coronaviruses (CoVs) 229E, NL63, OC43, and HKU1 cause mild, self-limiting respiratory tract infections, while the more virulent SARS-CoV-1, MERS-CoV, and SARS-CoV-2 have caused severe outbreaks. ... -
Genetic lesions within the 3a gene of SARS-CoV
Tan, Timothy H.P.; Barkham, Timothy; Fielding, Burtram C.; Chou, Chih-Fong; Shen, Shuo; Lim, Seng Gee; Hong, Wanjin; Tan, Yee-Joo (BioMed Central, 2005)A series of frameshift mutations within the 3a gene has been observed in culture-derived severe acute respiratory syndrome coronavirus (SARS-CoV). We report here that viral RNA from clinical samples obtained from SARS-CoV ... -
The genetic organisation of a 2,966 basepair DNA fragment of a Single Capsid Nucleopolyhedrovirus isolated from Trichoplusia ni
Fielding, Burtram C.; Khan, Sehaam; Wang, Weizhou; Kruger, Courtney; Abrahams, Rayaana; Davison, Sean (Springer Verlag, 2002)In order to investiagte the genomic organization of the Trichoplusia ni Single Capsid Nucleoplyhedrovirus (TnSNPV) , a 2,966 base pairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading ... -
Human coronavirus NL63 open reading frame 3 encodes a virion-incorporated N-glycosylated membrane protein
Müller, Marcel A.; van der Hoek, Lia; Voss, Daniel; Bader, Oliver; Lehmann, Dörte; Schulz, Axel R.; Kallies, Stephan; Suliman, Tasnim; Fielding, Burtram C.; Drosten, Christian; Niedrig, Matthias (BioMed Central, 2010)Background: Human pathogenic coronavirus NL63 (hCoV-NL63) is a group 1 (alpha) coronavirus commonly associated with respiratory tract infections. In addition to known non-structural and structural proteins all coronaviruses ... -
Human coronavirus research: 20 years since the SARS-CoV outbreak
Fielding, Burtram C. (Frontiers Media, 2022)This Editorial introduces 10 articles published in a Special Issue highlighting human coronavirus (hCoV) research on the twentieth anniversary of the outbreak of severe acute respiratory syndrome (SARS) in late 2002. ... -
Human coronaviruses: counteracting the damage by storm
Schoeman, Dewald; Fielding, Burtram C. (MDPI Viruses, 2021)Over the past 18 years, three highly pathogenic human (h) coronaviruses (CoVs) have caused severe outbreaks, the most recent causative agent, SARS-CoV-2, being the first to cause a pandemic. Although much progress has been ... -
Identification and characterization of the Trichoplusia ni Single Capsid Nuclear Polyhedrosis Virus p10 gene
Fielding, Burtram C.; Davison, Sean (Springer Verlag, 2000)The p10 gene was identified and characterized from the Trichoplusia ni single capsid nuclear polyhedrosis virus (TniSNPV). The p10 open reading frame (ORF) sequence was identified following sequencing of the ends of the ... -
Identification, sequence analysis, and phylogeny of the immediate early gene 1 of the trichoplusia ni single nucleocapsid polyhedrosis virus
Wang, Weizhou; Leat, Neil; Fielding, Burtram C.; Davison, Sean (Springer Verlag, 2001)Substantial research has been conducted on the immediate early 1 (ie-1) genes from the prototype baculovirus Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) and the Orgyia pseudotsugata multicapsid ... -
Leptin deficiency, caused by malnutrition, makes you susceptible to sars-cov-2 infection but could offer protection from severe Covid-19
Schoeman, Dewald; Fielding, Burtram C. (American Society for Microbiology, 2021)In much of the developing world, severe malnutrition is the most prevalent cause of immunodeficiency and affects up to 50% of the population in some impoverished communities. As yet, we do not know how severe acute ...