Browsing Research Articles (Biotechnology) by Title
Now showing items 143-162 of 233
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A label-free gold nanoparticles-based optical aptasensor for the detection of retinol binding protein 4
(MDPI, 2022)Retinol-binding protein 4 (RBP4) has been implicated in insulin resistance in rodents and humans with obesity and T2DM, making it a potential biomarker for the early diagnosis of T2DM. However, diagnostic tools for ... -
Large Scale Screening of Southern African Plant Extracts for the Green Synthesis of Gold Nanoparticles Using Microtitre-Plate Method
(Molecules, 2016-11)The preparation of gold nanoparticles (AuNPs) involves a variety of chemical and physical methods. These methods use toxic and environmentally harmful chemicals. Consequently, the synthesis of AuNPs using green chemistry ... -
A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye
(Future Science Group, 2008)We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated ... -
The macrophage response to mycobacterium tuberculosis and opportunities for autophagy inducing nanomedicines for Tuberculosis therapy
(Frontiers Media S.A., 2021)The major causative agent of tuberculosis (TB), i.e., Mycobacterium tuberculosis (Mtb), has developed mechanisms to evade host defense responses and persist within host cells for prolonged periods of time. Mtb is also ... -
Mapping and characterisation of the sorghum cell suspension culture secretome
(Academic Journals, 2011)Here we reported the first secretomic study of sorghum (Sorghum bicolor), a naturally drought tolerant cereal crop. In this study, we used a gel-based proteomic approach in combination with mass spectrometry to separate ... -
Meat trade: need for international standardization?
(Elsevier, 2013)Extensive substitution and undeclared species have been recently detected in meat products in South Africa, Europe and Asia. Here we review the methodologies utilized in the identification of species in red meat products ... -
Mechanistic insight into the anti-alternaria activity of bimetallic zinc oxide and silver/zinc oxide nanoparticles
(Elsevier Ltd, 2024)Alternaria alternata is an opportunistic phytopathogen that negatively impact the growth and production of a wide variety of host plants. In this study, we evaluated the antifungal potential of biogenic ZnO, and ... -
Methoxylated Flavonols and ent-Kaurane Diterpenes from the South African Helichrysum rutilans and Their Cosmetic Potential
(Multidisciplinary Digital Publishing Institute (MDPI, 2023)Abstract: Chromatographic fractionation of a methanol extract of Helichrysum rutilans afforded seven known compounds. The isolated compounds were identified as 5,7,8-trihydroxy-3,6-dimethoxyflavone8-O-2-methyl-2-butanoate ... -
Methoxylated flavonols and ent-kaurane diterpenes from the south african helichrysum rutilans and their cosmetic potential
(MDPI [Commercial Publisher], 2023)Chromatographic fractionation of a methanol extract of Helichrysum rutilans afforded seven known compounds. The isolated compounds were identified as 5,7,8-trihydroxy-3,6-dimethoxyflavone-8-O-2-methyl-2-butanoate (C-1), ... -
Methylenetetrahydrofolate Reductase Polymorphism (rs1801133) and the Risk of Hypertension among African Populations: A Narrative Synthesis of Literature
(MDPI, 2022)In this review, we have gathered and analyzed the available genetic evidence on the association between the methylenetetrahydrofolate reductase gene (MTHFR), rs1801133 and the risk of Hypertension (HTN) in African ... -
Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation
(Environmental Pollution, 2023)Petroleum hydrocarbons and heavy metals are some of the most widespread contaminants affecting marine ecosystems, urgently needing effective and sustainable remediation solutions. Microbial-based bioremediation is gaining ... -
Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation
(Elsevier, 2023)Petroleum hydrocarbons and heavy metals are some of the most widespread contaminants affecting marine ecosystems, urgently needing effective and sustainable remediation solutions. Microbial-based bioremediation is gaining ... -
MicroRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing
(PloS one, 2022)The growing demands on protein producers and the dwindling available resources have made Hermetia illucens (the black soldier fly, BSF) an economically important species. Insights into the genome of this insect will better ... -
MicroRNA-based regulation of Aurora A kinase in breast cancer
(Impact Journals, 2020)The involvement of non-coding RNAs (ncRNAs) in cellular physiology and disease pathogenesis is becoming increasingly relevant in recent years specifically in cancer research. Breast cancer (BC) has become a health concern ... -
Mitochondrial dysfunction and diabetes in South Africa: A review
(Elsevier B.V., 2024)Type 2 diabetes mellitus (T2DM) is a growing public health concern globally, and more so in developing countries like South Africa where it is characterized by an alarmingly high prevalence. Emerging evidence suggests that ... -
Modulation of antioxidant enzyme activities and metabolites ratios by nitric oxide in short-term salt stressed soybean root nodules
(Elsevier, 2013)Several abiotic factors cause molecular damage to plants either directly or through the accumulation of reactive oxygen species such as hydrogen peroxide (H2O2). We investigated if application of nitric oxide (NO) donor ... -
Molecular adaptation mechanisms employed by ethanologenic bacteria in response to lignocellulose-derived inhibitory compounds
(Ivyspring, 2013)Current international interest in finding alternative sources of energy to the diminishing supplies of fossil fuels has encouraged research efforts in improving biofuel production technologies. In countries which lack ... -
Molecular and epidemiological analyses of sheeppox outbreaks in Russia from 2013 to 2021
(Wiley-Hindawi, 2023)Sheeppox (SPP) is a transboundary infectious disease that affects sheep and goats, leading to significant losses in countries with extensive small ruminant breeding programs. While sporadic SPP outbreaks have been observed ... -
Molecular application of aptamers in the diagnosis and treatment of cancer and communicable diseases
(MDPI, 2018)Cancer and infectious diseases such as Ebola, HIV, tuberculosis, Zika, hepatitis, measles and human schistosomiasis are serious global health hazards. The increasing annual morbidities and mortalities of these diseases ... -
Morpho-physiological, biochemical, and genetic responses to salinity in medicago truncatula
(MDPI, 2021)We used an integrated morpho-physiological, biochemical, and genetic approach to investigate the salt responses of four lines (TN1.11, TN6.18, JA17, and A10) of Medicago truncatula. Results showed that TN1.11 exhibited a ...